Examinando por Materia "Chromatography"

Seleccione resultados tecleando las primeras letras
Mostrando 1 - 2 de 2
  • Miniatura
    PublicaciónAcceso abierto
    Determinación de etanol en sangre en individuos examinados por dos métodos analíticos en el laboratorio central de la Dirección de Criminalística de la Policía Nacional del Perú. Lima 2023
    (Universidad Privada Norbert Wiener, 2024-02-27) Puchoc Quispe, Sayuri Yasumi; Vega Salazar, Antony; Ramos Jaco, Antonio Guillermo
    The objective of this study was to validate the methods for blood alcohol analysis using GC-FID and UV-VIS and to determine the differences between ethanol concentrations in blood samples from individuals tested at the Central Laboratory of DIRCRI-PNP. The methodology employed was the hypothetical-inductive method, with a quantitative approach, basic and descriptive, longitudinal, and of comparative level. The performance characteristics evaluated in the validation were: selectivity, sensitivity, linearity, accuracy, precision, and robustness, using blood samples with ethanol. For the determination of ethanol in individuals, 240 biological blood samples from the Toxicology Service – DIRCRI-PNP Sampling were processed and analyzed using the GC-FID and UV-VIS methods. Data processing for validation was performed using ANOVA, linear regression, F-test, and Student's t-test applied to the mentioned methods. The performance characteristics of the GC-FID and UV-VIS methods were: detection limits of 0.00191 g/L and 0.0123 g/L; quantification limits of 0.00636 g/L and 0.0411 g/L; accuracy as % recovery of 96.59–100.16% and 86.17–101.16%; repeatability as coefficient of variation of 2.01% and 26.47%; intermediate precision as coefficient of variation of 1.88% and 22.11%. Conclusion: The GC-FID method cannot be interchangeable or replaceable by UV-VIS due to the high variability in the performance characteristics of the methods.
  • Miniatura
    PublicaciónAcceso abierto
    Evaluación del nivel de ocratoxina A en las fases de post cosecha de Theobroma cacao L. “cacao trinitario” y chocolates artesanales en el distrito de Pisana – San Martin, 2018
    (Universidad Privada Norbert Wiener, 2022-02-01) Ariza Aguayo, Abigail Janet; Laupa Lukaña, Edith Esperanza; Chávez Flores, Juana Elvira
    Ochratoxin A is a nephrotoxic and carcinogenic mycotoxin capable of causing harmful effects to humans and animals, and it is found in many foods, including cocoa and its derivatives. The objective of this study was to determine and quantify the levels of ochratoxin A in Theobroma cacao L. in its post-harvest stages and in artisanal chocolates from the district of Pisana, San Martin. The methodology involved an extraction process using immunoaffinity columns and quantification by High-Performance Liquid Chromatography (HPLC). The cacao and artisanal chocolate samples were collected in the Pisana district of the San Martin department in 2018. Detection was performed by fluorescence, at an excitation wavelength of 333 nm and an emission wavelength of 460 nm. The results from the stored cacao samples showed values of 0.938 and 0.935 ppb of ochratoxin A for injection 1 and injection 2, respectively. We concluded that the values from the cacao samples did not exceed the detection limit of 0.798 µg/kg and that both injections did not surpass the limits permitted by international legislation. This study highlights the importance of the traceability of potentially harmful compounds in the production chain of high-demand foods, both nationally and internationally, to ensure their quality.