Examinando por Materia "Coliformes"

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Desconocido
Análisis bacteriológico del agua de la fuente de abastecimiento y de jeringa triple de las unidades dentales de clínicas odontológicas en Tarma (Junín), período octubre 2012–febrero 2013
(Universidad Privada Norbert Wiener, 2013) Liñán Trujillo, José Luis; Reynozo Gálvez, Cecilia Elisa; Chávez Fernández, Ana María
In the determination of the bacteriological quality of the water from the supply source and the three-way (air–water) syringe used in the dental units of dental clinics in the city of Tarma (Junín), sampling was conducted in 25 dental clinics with continuous service, for a total of 30 water samples: 5 from the supply source and 25 from the three-way syringe. Counts were made of heterotrophic bacteria, total coliforms, thermotolerant coliforms, Escherichia coli and the presence of Pseudomonas aeruginosa, using the membrane filtration method based on Standard Methods for the Examination of Water and Wastewater (AWWA, APHA and WEF), and following the guidelines of the Peruvian Ministry of Health (MINSA) Regulation on Water Quality for Human Consumption. Culture media and glassware were prepared and sterilized at Félix Mayorca Soto Hospital. The analysis of potable water samples was carried out in the laboratory of the Pharmacy technical programme at Instituto Santa Lucía in the city of Tarma (Junín). At all times, the work was supported by specialized professionals in the Clinical Laboratory area. It was observed that the five supply-source water samples showed absence of total coliforms, thermotolerant coliforms, Pseudomonas aeruginosa, Escherichia coli, and fewer than 500 CFU of heterotrophic bacteria. In contrast, analysis of the 25 three-way syringe water samples showed the presence of total coliforms in 88% of the dental offices, fecal coliforms in 32%, heterotrophic bacteria in 20%, Pseudomonas aeruginosa in 16%, and Escherichia coli in 8%. Therefore, only three water samples from the dental clinics complied with the parameters established by MINSA.
Acceso abierto
Determinación de la calidad microbiológica e inocuidad del agua potable para consumo de los dispensadores de las boticas y farmacias del distrito de Breña en el departamento de Lima, mayo-junio de 2017
(Universidad Privada Norbert Wiener, 2017) Bobadilla Poquioma, Carlos Yoel; Hurtado Cahuana, José Luis; Guevara Vicaña, Rita Aurora
Water intended for consumption, the preparation of food and beverages, or personal hygiene must not contain any human pathogens. This can be achieved by selecting high-quality water sources, effectively treating and decontaminating water polluted by human or animal feces or other substances, and protecting it from contamination during distribution to the user. Therefore, it is necessary to determine the presence of microorganisms that indicate water safety (total coliforms) and those that indicate water microbiological quality (Heterotrophs). This was carried out on samples from 67 pharmaceutical establishments in the Breña district during May–June 2017. The determination of coliform microorganisms was performed by the multiple tube method, and the determination of heterotrophic microorganisms was performed by the pour plate count method. The results were compared with the standards established in the Regulation on Drinking Water Quality: D.S. N° 031-2010-SA / Ministry of Health. General Directorate of Environmental Health – Lima: Ministry of Health; 2011. 4.5% of the samples did not comply with the established parameters (Heterotrophs), as the maximum permissible limit according to current national standards is 500 CFU/mL of sample.
Acceso abierto
Determinación de la calidad microbiológica en bebidas no carbonatadas, jarabeadas y no jarabeadas de venta libre en el distrito del Cercado de Lima, 2023
(Universidad Privada Norbert Wiener, 2024-05-05) Torrejón Quezada, Fanny Lissette; Moya Salazar, Jeel Junior
La calidad microbiológica alimentaria es importante para asegurar la salud humana, sin xembargo, hay productos de venta callejera que no tienen un control adecuado y por tanto representan un importante riesgo sanitario. El objetivo de este estudio fue determinar la calidad microbiológica de bebidas no carbonatadas, jarabeadas y no jarabeadas de venta libre en el distrito del Cercado de Lima, 2023.
Acceso abierto
Determinación de la calidad sanitaria e inocuidad de salsas picantes servidas en establecimientos comerciales de la urbanización Santa Anita en el distrito de Santa Anita – Lima abril 2018
(Universidad Privada Norbert Wiener, 2019-06-26) Rosales Ñaupari, Ketty Inés; Guevara Vicaña, Rita Aurora
The objective of this study was to evaluate the level of sanitary quality and safety of hot sauces served in commercial establishments in the Santa Anita Urbanization in the Santa Anita District. For this purpose, a total of 30 hot sauce samples were analyzed: 10 from market stalls (0-fork category), 10 from restaurants (1-fork category), and 10 from restaurants (2-fork category). The sampling was non-probabilistic and by convenience. Method: The 3M Petrifilm Plates for Mold and Yeast Count and for Total Coliform Count (AOAC 991.14) were used. For data interpretation, the limits established by Sanitary Technical Standard NTS No. 071 – MINSA/DIGESA - V.01 were applied. Results: Of the establishments studied, 1 out of 10 (10.00%) in the 0-fork category, 2 out of 10 (20.00%) in the 1-fork category, and 2 out of 10 (20.00%) in the 2-fork category complied with the microbiological criteria. Conclusion: The commercial establishments in the Santa Anita Urbanization have low sanitary quality and safety in the hot sauces served, according to the permissible values of microbiological indicators established in the aforementioned standard.
Acceso abierto
Determinación microbiológica de coliformes en alimentos de venta ambulatoria en el mercado de La Victoria – Chiclayo Perú año - 2024
(Universidad Privada Norbert Wiener, 2025-08-22) Carlos Rodriguez, Felicia; Pantoja Moreno, Fernando Belardino; Pineda Pérez, Neuman Mario
Food hygiene and safety are fundamental aspects for protecting public health, especially in street vendor settings where conditions are often precarious. Street food sales, such as those in traditional markets, pose significant health risks due to poor hygiene practices during food preparation and handling, which can facilitate microbiological contamination and disease transmission. The objective of the research was to determine the incidence of coliforms in street vendors' food at the La Victoria market in Chiclayo. During 2024, the hygienic conditions of 15 food stalls were evaluated, and three groups of food samples (cevichocho, bread with cheese, and vegetable salad) will be randomly collected. The samples were analyzed in the authorized microbiology and parasitology laboratory of the Faculty of Biological Sciences at the Pedro Ruiz Gallo National University.The results showed that the stalls did not meet minimum standards for sanitation and proper handling. Microbiological analysis of the bread with cheese identified bacteria such as Enterobacter, Proteus, Escherichia, Streptococcus, Bacillus, and Staphylococcus; Escherichia, Bacillus, Enterobacter, Proteus, Yersinia, and Streptococcus were detected in the lupin ceviche; while Bacillus, Escherichia, Streptococcus, and Staphylococcus were found in the vegetable salad. It is concluded that the presence of these findings underscores the urgent need to implement sanitary control measures, training in good handling practices, and infrastructure improvements to ensure safe food and protect public health in the La Victoria market.
Acceso abierto
Evaluación de los parámetros de control obligatorio de la calidad del agua para consumo humano en los reservorios del centro poblado rural Río Seco – Cieneguilla, marzo–octubre, 2019
(Universidad Privada Norbert Wiener, 2020-07-21) Chacmana Asturima, Edgar Angel; Blas Manyari, Cristopher Isaac; Collanque Pinto, Jesús Daniel
The study of water quality has been evaluated by many national and international studies, as it is essential and vital for water intended for human consumption to have the necessary characteristics to ensure its risk-free consumption. The WHO, through its guidelines for drinking water quality, emphasizes prevention and sustainable access to drinking water for economic development. Locally, the General Directorate of Environmental Health (DIGESA) aligns with this, especially with its regulatory framework DS-031-2010-SA. The objective of this study was to evaluate the mandatory control parameters for the quality of water for human consumption in the reservoirs of the Rio Seco – Cieneguilla rural town center, from March to October 2019. The methodology used was a descriptive, cross-sectional, and non-experimental study with quantitative microbiological and physical analysis based on current regulations. The population consisted of 46 properties, and quality was evaluated according to the following parameters: Determination of total coliforms and Escherichia coli, and determination of physical parameters (pH, residual chlorine, turbidity, and color). An evaluation through surveys showed that 67.4% of properties did not continuously clean their reservoir and 28.3% did not purify the water they consumed. This improved after training was provided, with a predisposition to improve sanitary measures, resulting in 70% purifying their water and 80% continuously cleaning their reservoirs. Results: 97.8% (45 samples) tested positive for total coliforms, and 34.8% (16 samples) tested positive for Escherichia coli. These results were compared with the permissible parameters according to current drinking water quality regulations. Conclusion: The water consumed by the population does not comply with the mandatory microbiological parameters of the DS N° 031-2010 SA, DIGESA regulation.
Acceso abierto
Evaluación microbiológica de alimentos adquiridos en la vía pública del Cercado de Lima entre mayo de 2017 y junio de 2018
(Universidad Privada Norbert Wiener, 2019-01-16) Galarza Sánchez, Katherine Elayni; Parreño Tipián, Juan Manuel
The objective of this thesis was to determine the microbiological quality of food intended for human consumption in the district of Cercado de Lima between May 2017 and June 2018. The study was descriptive, observational, and cross-sectional. Ten food samples with heat treatment and ten without heat treatment were randomly selected and evaluated. Sample processing was carried out in the laboratories of Norbert Wiener University, where the incorporation count technique was used to determine the presence of mesophilic aerobic bacteria, total coliforms, and yeast-like fungi. The results were obtained and compared with the Sanitary Technical Standard NTS Nº 071 - MINSA/DIGESA - V.01. In the Colony-Forming Units (CFU) count, it was observed that both types of samples had the same percentage of mesophilic aerobic bacteria. However, the quantity of total coliforms found in foods without heat treatment was higher compared to those with heat treatment. A high percentage of yeast-like fungi was also found in both types of samples. It is concluded that foods without heat treatment presented elevated counts, which, in accordance with the Sanitary Technical Standard, makes them unfit for human consumption.