Examinando por Materia "Essential oils"

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(Universidad Privada Norbert Wiener, 2015) Cerga Rodriguez, Leslie Alexandra; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of essential oils from Cinnamomum zeylanicum (cinnamon) and Syzygium aromaticum (clove) in comparison to 2% Chlorhexidine Gluconate against Enterococcus faecalis strains. The study was experimental, prospective, longitudinal, and analytical. The essential oils from these plants were obtained using the steam distillation method, utilizing the bark of Cinnamomum zeylanicum (cinnamon) and dried buds of Syzygium aromaticum (clove) at concentrations of 1%, 5%, 25%, 50%, 75%, and 100%. For the experiment, Enterococcus faecalis (ATCC 29212) was reactivated and then cultured on Petri dishes (15 cm in diameter) containing Mueller Hinton agar with 6 mm diameter wells, where approximately 100 µl of the essential oils were added, compared to 2% Chlorhexidine Gluconate as a positive control and Tween 20 as a negative control. The plates were incubated at 37°C to check for bacterial viability and media sterility, and the inhibition zones were measured using a Vernier caliper at 24 and 48 hours. In vitro sensitivity testing yielded the following results: the antimicrobial effect of Cinnamomum zeylanicum (cinnamon) essential oil at its minimum effective concentration (1%) was greater than the antimicrobial effect of Syzygium aromaticum (clove) essential oil at its minimum effective concentration (25%). Additionally, a greater antimicrobial effect was observed with the positive control, 2% Chlorhexidine Gluconate.
Acceso abierto
Comparison of antibacterial activity of essential oils from Schinus molle L. (molle) and Thymus vulgaris (thyme) with chlorhexidine gluconate 0.12% against Porphyromonas gingivalis: in vitro study
(Universidad Privada Norbert Wiener, 2019-01-17) Neira Jara, Vanessa Evelin; Salcedo Rioja, Mercedes Rita
This research study was conducted in vitro with the objective of comparing the antibacterial activity of essential oils from Schinus molle L. (molle) and Thymus vulgaris (thyme) with 0.12% chlorhexidine gluconate against Porphyromonas gingivalis. This experimental study was carried out at the National University of San Marcos in the Microbiology Laboratory of the Faculty of Dentistry. The sample consisted of 40 Petri dishes where the essential oils Thymus vulgaris at 100% and 50%, Schinus molle L. at 100% and 50% were used, compared with 0.12% chlorhexidine gluconate, and dimethyl sulfoxide (DMSO) as a negative control. The disk diffusion method was used, and the dishes were incubated for 15 days at 37°C. The inhibition halos were measured only once at the time of measurement. Data analysis was processed using the Scheffé's Post Hoc statistical test. The results showed that, of the two oils, thyme had the greatest effect on Porphyromonas gingivalis, while molle had the least effect. Both thyme and molle essential oils exhibited greater antibacterial activity than 0.12% chlorhexidine gluconate.