Examinando por Materia "Microscopía"

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Acceso abierto
Comparative study of automated leukocyte differential count and reference method in the hematology laboratory of the National Institute of Neoplastic Diseases, 2021
(Universidad Privada Norbert Wiener, 2021-12-09) Chavez Anastacio, Dorys Isabel; Champa Guevara, Cesar Alfonso
The microscopic evaluation of a peripheral blood smear is a diagnostic tool in the hematology laboratory. Automated analyzers mark results for a significant proportion of samples and require confirmation through smear review. Therefore, each clinical laboratory needs to verify the reliability of the automated white blood cell differential count (WBC DC) and compare it with the reference method. Objective: To determine if there is a significant difference between the automated white blood cell differential count and the reference method in the Hematology Laboratory of the National Institute of Neoplastic Diseases, 2021. Methods: A prospective cross-sectional study will be conducted at the Hematology Laboratory from October to December 2021. Data will be analyzed using Excel templates, and the following will be performed: (1) For determining the association between two variables with a normal distribution, Pearson correlation will be used, while Spearman’s correlation will be applied if the data does not follow a normal distribution. (2) To compare methods, the standard error of proportion with 95% confidence intervals will be calculated for each cell type. (3) For comparing paired means, statistical significance will be determined using a p-value < 0.05 (Student’s t-test). (4) For analyzing bias (systematic error), the regression equation will be considered.
Acceso abierto
Microscopic characterization of garment textile openings in the investigation of crimes against life, body and health
(Universidad Privada Norbert Wiener, 2021-12-09) Pastor Matías, Hannz Alexis; Verástegui Ramírez, Ibeth María; Ascarza Gallegos, Justo Angelo
The objective of the study was to characterize holes found in textile openings using optical microscopy. The fabric type was considered. These fabrics were perforated according to the type of damage that could be inflicted on the textile (cut, tear, and burn). The created hole was placed on a slide with a fixative. Observations were made using 40x and 100x objectives. The characteristics of each sample were recorded in a validated instrument for documentation purposes. It was determined that for cuts, a serrated fiber finish was obtained in animal, vegetable, and synthetic fabrics, with a prevalence of 87%, 93%, and 93%, respectively, at 40x magnification; and 100% for all at 100x magnification. For tearing, the prevalence of an irregular finish was observed for animal, vegetable, and synthetic fibers at 80%, 87%, and 93%, respectively, at 40x magnification; and 100% for all at 100x. For burning, a fused finish was prevalent for all fabrics at both magnifications, with 100% at 40x and 100x. The conclusion is that microscopic characteristics help identify the mechanism that caused the damage to the fabric.