Examinando por Materia "Phaseolus"

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Acceso abierto
Actividad anti-tirosinasa y efecto fotoprotector del extracto etanólico del tegumento de dos variedades de Phaseolus vulgaris L., ñuña roja y negra
(Universidad Privada Norbert Wiener, 2020-12-01) Mera Santa Cruz, Ermitanio; Papuico Sanchez, Liliana Eulalia; Chávez Flores, Juana Elvira
The objective of this study was to determine the anti-tyrosinase activity and evaluate the photoprotective effect of the ethanolic extract from the tegument of two varieties of Phaseolus vulgaris L., red and black 'ñuña'. Methodology: Anti-tyrosinase activity was determined with the thin-layer chromatographic method using a 0.05% Kojic acid standard. For the photoprotective effect, 35 female Mus musculus Balb/C53 strain mice, seven weeks old, were divided into five experimental groups. UV-C radiation was applied to their backs for 30 minutes once a day for one month. Acute photodamage was evaluated using the Draize scale, and photoaging was evaluated using the Glogau scale. Results: Secondary metabolites such as polyphenols and flavonoids were found. The extract was not toxic in mice via oral and dermal administration at a limit dose of 5000 mg/kg. It has anti-tyrosinase activity, and when applied in a 5% gel, the red variety showed a greater effect. It also has a photoprotective effect in mice subjected to UVC radiation. Conclusion: The ethanolic extract from the tegument of the black 'ñuña' at 5% in gel has a photoprotective effect.
Acceso abierto
Contenido de polifenoles totales, capacidad antioxidante, composición proximal y digestibilidad proteica de las variedades roja y negra de ñuñas nativas del Perú
(Universidad Privada Norbert Wiener, 2022-08-18) Cano Salvador, Geovanny Leonardo; Collantes Llacza, Adela Marlene
Phaseolus vulgaris L. is one of the main protein sources in the world, also containing minerals, vitamins, and carbohydrates, making it one of the essential resources in the human diet. The objective of this study was to determine the total polyphenol content, antioxidant capacity, proximate composition, and protein digestibility of the red and black varieties of native Peruvian ñuñas. The total polyphenol content was quantified using the Swaint and Hills method, expressed in mg of gallic acid equivalent per 100 g of sample; antioxidant capacity was measured using the Arnao, Marino, and Cano method, expressed in micromoles of Trolox equivalent per 100 g of sample; proximate composition and digestible protein were measured using the Max Becker method, expressed in grams per 100 g of sample. It was found that the total polyphenol content is 41.87% higher in the red variety than in the black variety; the antioxidant capacity of the black variety was 35.13% higher than the red variety; the proximate composition is similar in both varieties, balanced and suitable for a nutritious diet; and the protein digestibility of the red variety is 2.47% higher than the black variety of native Peruvian ñuñas. It is concluded that ñuña is a functional food due to its nutritional and antioxidant properties, with good digestible protein content and antioxidant activity.
Acceso abierto
Identificación y cuantificación de aminoácidos esenciales en Cicer Arietinum L. garbanzo y Phaseolus lunatus L. pallar por cromatografía líquida de alta performance (HPLC)
(Universidad Privada Norbert Wiener, 2021-01-18) Ccala Sucasaca, Jackelin; Ramirez Carrasco, Shaila; Félix Véliz, Luis Miguel Visitación
Chickpeas and lima beans are part of the legume family. They are important from a nutritional point of view. The objective of this research was to identify and quantify the essential amino acids in Cicer arietinum L. (chickpea) and Phaseolus lunatus L. (lime beans), using drying, pulverizing and degreasing with petroleum ether as a methodology, hydrolyzing it with 6N HCl hydrochloric acid, obtaining the protein extract of Cicer arietinum L. (chickpea) and Phaseolus lunatus L. (lime beans). The results were identified and quantified with high performance chromatography, in Cicer arietinum L. "chickpea" the following amino acids were found: Alanine (0.007 mg/100 mg), Glycine (0.929 mg/100 mg), Methionine (0.001 mg/100 mg), Valine (0.001 mg/100 mg), Phenylalanine (0.033 mg/100 mg) and Lysine (1.027 mg/100 mg); and in Phaseolus lunatus L. “pallar” the following were found: Alanine (0.008 mg/100 mg), Glycine (0.330 mg/100 mg), Methionine (0.001 mg/100 mg), Valine (0.003 mg/100 mg), Phenylalanine (0.033 mg/100 mg) and Lysine (0.934 mg/100 mg). With the data obtained in this research, the conclusion is that the legumes studied do contain essential amino acids such as: Methionine, Valine, Phenylalanine and Lysine; which can be part of the daily diet due to their high lysine content.
Acceso abierto
Identificación y cuantificación de aminoácidos esenciales en Vigna unguiculata (frejol castilla) y Phaseolus vulgaris (frejol guinda) por cromatografía líquida de alta performance (HPLC)
(Universidad Privada Norbert Wiener, 2018-09-27) Flores Hilario, Elsa; Montoya Navarro, Kelly Janys; Félix Véliz, Luis Miguel Visitación
The present research aimed to identify the essential amino acids in Vigna unguiculata and Phaseolus vulgaris. The samples were dried and pulverized, followed by defatting with 500 mL of petroleum ether using the Soxhlet method. Hydrolysis was carried out with 6N HCl. The identification of essential amino acids in Vigna unguiculata and Phaseolus vulgaris was performed using High-Performance Liquid Chromatography (HPLC). The results showed that Vigna unguiculata contained valine (1.02 mg/100 mg), glycine (8.75 mg/100 mg), leucine (1.01 mg/100 mg), lysine (0.64 mg/100 mg), and methionine (1.05 mg/100 mg), while Phaseolus vulgaris contained valine (1.79 mg/100 mg), glycine (5.49 mg/100 mg), tryptophan (1.33 mg/100 mg), leucine (1.08 mg/100 mg), and lysine (1.31 mg/100 mg). Based on these findings, it is recommended to include legumes as part of a daily diet.