Examinando por Materia "Plant Extracts"

Mostrando 1 - 20 de 54

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Actividad analgésica del extracto etanólico de la raíz Vallea stipularis L.F. "Chuillur" en ratones
(Universidad Privada Norbert Wiener, 2018-09-18) Picho Hurtado, Katheryn Elizabeth; Chávez Flores, Juana Elvira
The investigation was carried out on the plant species Vallea stipularis L.f. “Chuillur,” located in the district of Tamburco-Abancay-Apurímac, traditionally used by the local population as medicine for the treatment of scurvy, as a cicatrizant, for gastritis, rheumatism, drastic purgative, and analgesic. Objective: To verify the analgesic activity of the ethanolic extract of the root of Vallea stipularis L.f. “Chuillur” in mice. Methods: The method of abdominal writhing induced by 0.8% acetic acid was employed. Forty-nine albino mice of the species Mus musculus strain Balb/C53/CNPB, of both sexes, were randomly distributed into seven groups. Ethanolic extracts at doses of 50, 100, and 200 mg/kg were used, as well as the standards Paracetamol Q.P. 300 mg/kg and Tramadol Hydrochloride Q.P. 40 mg/kg. These were administered orally, and 30 minutes later, 0.8% acetic acid was administered intraperitoneally. The animals were immediately observed for 20 minutes. Results: All groups showed a significant reduction (p value = 0.000), obtaining differentiated analgesic effects. The maximum effect was achieved with the 200 mg/kg extract, with 70% inhibition, surpassing Paracetamol (55%). Conclusions: It is concluded that the ethanolic extract of the root of the plant species Vallea stipularis L.f. “Chuillur” has analgesic effect.
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Actividad analgésica y antiinflamatoria del extracto etanólico de la raíz de Taraxacum officinale Wigg “diente león” en ratones (Mus musculus)
(Universidad Privada Norbert Wiener, 2018-05-07) Castañeda Cuadros, Renzo Gerardo; Miranda Velarde, Anthony Boris; Chávez Flores, Juana Elvira
The objective of the study was to determine the analgesic and anti-inflammatory activity of the ethanolic extract from the root of Taraxacum officinale Wigg, "dandelion," in mice. The root was macerated for 7 days in 70% ethanol. Solubility and qualitative analyses were performed, and the extract was determined to be soluble in polar solvents and to contain flavonoids and alkaloids. To determine the analgesic activity, the 0.8% glacial acetic acid-induced abdominal writhing model was used on 49 Balbin/C53/CNPB strain mice. The groups were a negative control (distilled water), a positive control (0.8% acetic acid), the ethanolic extract at 25, 50, and 100 mg/kg, and comparative standards of paracetamol (300 mg/kg) and tramadol (40 mg/kg). To determine the anti-inflammatory effect, the subplantar edema method was used, with dexamethasone (4 mg/kg) and diclofenac (50 mg/kg) as standard drugs. The evaluation of the extract's analgesic activity showed an effective dose of 100 mg/kg with 69% writhing inhibition. The anti-inflammatory activity was effective at a dose of 25 mg/kg, with an inhibition percentage evolving from 56% to 99% over a six-hour period.
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Actividad analgésica y antiinflamatoria del extracto etanólico de las flores de Bidens andicola H.B.K. "quiquo"
(Universidad Privada Norbert Wiener, 2018-03-01) Huarcaya Huarcaya, Liliana; Sotelo Saravia, Nancy Avelina; Chávez Flores, Juana Elvira
The objective of this study was to determine the analgesic and anti-inflammatory activities of the ethanolic extract of Bidens andicola H.B.K “quiquo” flowers. For the analgesic activity, the modified Koster et al. model was employed, and the study groups were: negative control (distilled water), positive control (0.8% glacial acetic acid), ethanolic extract of Bidens andicola H.B.K. “quiquo” flowers at 50, 100, and 200 mg/kg, paracetamol 300 mg/kg, and tramadol 40 mg/kg. For the anti-inflammatory activity, the plantar edema method with 1% albumin was used, and the groups were: negative control (distilled water), ibuprofen 400 mg/kg, dexamethasone 1 mg/kg, and the ethanolic extract at 200, 400, and 600 mg/kg. The results show that the ethanolic extract of Bidens andicola H.B.K. “quiquo” flowers at 100 mg/kg (65%) and 200 mg/kg (70%) exhibits analgesic activity, lower than tramadol (91%) but comparable to paracetamol (81%). Regarding the anti-inflammatory activity, the ethanolic extract at 200, 400, and 600 mg/kg demonstrated efficacy, with the 600 mg/kg dose showing the greatest anti-inflammatory effect (100%), similar to dexamethasone and ibuprofen. It was confirmed that the ethanolic extract of Bidens andicola H.B.K. “quiquo” flowers possesses both analgesic and anti-inflammatory activities.
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Actividad analgésica y antiinflamatoria del extracto hidroalcólico de hojas y tallos de Leucheria daucifolia (D. Don.) Crisci "Churoq wasin" en ratones
(Universidad Privada Norbert Wiener, 2018-12-07) Soto Requez, Yvon Carla; Ruiz Rondinel, Sandra; Chávez Flores, Juana Elvira
The present study aimed to evaluate the analgesic and anti-inflammatory activity of the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin”. For the analgesic activity, the model of abdominal writhing induced by 0.8% acetic acid was employed; 49 mice were used, and the evaluated groups were: negative control (distilled water), positive control (0.8% acetic acid), hydroalcoholic extract at 50, 100 and 200 mg/kg, compared with paracetamol 300 mg/kg and tramadol 40 mg/kg. For the anti-inflammatory effect, the auricular edema method was used with an irritant agent (6% xylene diluted in acetone) applied to the mouse auricle. A total of 49 albino mice were used, and the study groups were: vaseline cream, creams prepared with the hydroalcoholic extract at 0.5, 1 and 2%, compared with diclofenac 1% cream and hydrocortisone 1% cream. The results show that the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin” at a dose of 100 mg/kg presents analgesic activity with an inhibition percentage of writhing of 62%, not surpassing tramadol (86%) but comparable to paracetamol (76%). Regarding anti-inflammatory activity, the cream with 2% extract presented an inhibition percentage of inflammation of 48.7%, which is comparable to hydrocortisone 1% and diclofenac 1%. It was confirmed that the hydroalcoholic extract possesses both analgesic and anti-inflammatory activity in mice.
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Actividad anti-tirosinasa y efecto fotoprotector del extracto etanólico del tegumento de dos variedades de Phaseolus vulgaris L., ñuña roja y negra
(Universidad Privada Norbert Wiener, 2020-12-01) Mera Santa Cruz, Ermitanio; Papuico Sanchez, Liliana Eulalia; Chávez Flores, Juana Elvira
The objective of this study was to determine the anti-tyrosinase activity and evaluate the photoprotective effect of the ethanolic extract from the tegument of two varieties of Phaseolus vulgaris L., red and black 'ñuña'. Methodology: Anti-tyrosinase activity was determined with the thin-layer chromatographic method using a 0.05% Kojic acid standard. For the photoprotective effect, 35 female Mus musculus Balb/C53 strain mice, seven weeks old, were divided into five experimental groups. UV-C radiation was applied to their backs for 30 minutes once a day for one month. Acute photodamage was evaluated using the Draize scale, and photoaging was evaluated using the Glogau scale. Results: Secondary metabolites such as polyphenols and flavonoids were found. The extract was not toxic in mice via oral and dermal administration at a limit dose of 5000 mg/kg. It has anti-tyrosinase activity, and when applied in a 5% gel, the red variety showed a greater effect. It also has a photoprotective effect in mice subjected to UVC radiation. Conclusion: The ethanolic extract from the tegument of the black 'ñuña' at 5% in gel has a photoprotective effect.
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Actividad antibacteriana de los extractos de acetato de etilo y acetona del zumo de Punica granatum L. "granada"
(Universidad Privada Norbert Wiener, 2017-11-13) Escobar Figueroa, Bernardita Jaquely; Quispe Quispe, Lidia; Félix Véliz, Luis Miguel Visitación
Punica granatum L. (“pomegranate”) has been used in traditional medicine since ancient times for the treatment of various diseases. Several studies have demonstrated its antioxidant, anti-inflammatory, antiparasitic, and antifungal properties, mainly from hydroalcoholic extracts of the peel. However, few studies have investigated pomegranate juice extracts using acetone and ethyl acetate. The aim of this research was to evaluate the antibacterial activity of acetone and ethyl acetate extracts from pomegranate juice. Antibacterial activity was assessed using the disk diffusion method. Four concentrations (200 mg/mL, 300 mg/mL, 400 mg/mL, and 500 mg/mL) of each extract were tested against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. Inhibition zones were qualitatively determined according to the Durafford scale for in vitro inhibitory effects in phytotherapy and CLSI antimicrobial standards. The results indicated that the acetone extract exhibited antibacterial activity against S. aureus and E. coli. Furthermore, a comparative analysis revealed that microbial inhibition was inversely proportional to the quantification of ellagic acid. These findings highlight the potential of pomegranate juice acetone extract as a natural antimicrobial source.
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Actividad antibacteriana frente a Cutibacterium acnes y antioxidante de los extractos hidroalcohólicos de las hojas, flores, tallo y raíz de Hypericum silenoides Juss "canchalagua"
(Universidad Privada Norbert Wiener, 2020-12-10) Moy Diaz, Brenda; Ortiz Arévalo, Yesenia Edith; Félix Véliz, Luis Miguel Visitación
The species Hypericum silenoides Juss "Canchalagua," native to the Cajamarca department, is a plant with various medicinal applications. The Objective of this research was to determine the antibacterial activity against Cutibacterium acnes and the antioxidant activity of the hydroalcoholic extracts from the leaves, flowers, stem, and root of Hypericum silenoides Juss, 'Canchalagua.' The Method employed consisted of preparing the hydroalcoholic extracts of the leaves, flowers, stem, and root of Hypericum silenoides Juss, followed by a solubility test and qualitative analysis to identify the metabolites. To determine the antibacterial activity against Cutibacterium acnes (ATCC 6919), the disk diffusion method was performed. For antioxidant activity, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical method was used for evaluation. Results: The microbiological assay showed that the positive control group presented a larger diameter of the inhibition halo compared to the plant species extracts studied. The hydroalcoholic extract of the flowers showed greater antibacterial activity compared to the extracts of the leaves, stem, and root. The hydroalcoholic extract of the leaves of Hypericum silenoides Juss, 'Canchalagua,' presented the highest antioxidant activity with an IC50 of 54.53 µg/mL, followed by the hydroalcoholic extract of the flowers with an IC50 of 57.05 µg/mL, compared to the control (Trolox) with a p < 0.05. Conclusion: The hydroalcoholic extracts of the leaves, flowers, stem, and root have antibacterial activity against Cutibacterium acnes and antioxidant activity.
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Actividad anticonvulsivante del extracto hidroalcoholico de raíz de Valeriana pinnatifida R. & P. "Valeriana"
(Universidad Privada Norbert Wiener, 2018-11-24) Anco Maximiliano, Yanet Soleda; Chávez Fernández, Ana María
Valeriana pinnatifida is a native plant of the Valerianaceae family, endemic to Peru, which grows on rocky slopes in the Andes. Traditionally, it has been used as a sedative, hypnotic, and tranquilizer. This study was initiated to analyze the metabolites present in the plant, determine its acute toxicity, and evaluate its anticonvulsant effect. The research was quasi-experimental. For the phytochemical study, a hydroalcoholic extract was obtained from the root of Valeriana pinnatifida R. & P., "valeriana," via maceration. Acute toxicity was determined at a limit dose of 2000 mg/kg using the OECD 423 protocol. The anticonvulsant effect was evaluated using an experimental model of pentylenetetrazol (PTZ)-induced seizures in mice. Five groups of six albino mice of the C53/CNPB strain were used: group 1 (saline control), group 2 (PTZ 80mg/kg), group 3 (diazepam 5mg/kg + PTZ), group 4 (EHVP 300mg/kg + PTZ), and group 5 (EHVP 400mg/kg + PTZ). The treatments were administered orally 30 minutes before the induction of seizures with PTZ. The results identified the secondary metabolites alkaloids and terpenes. The toxicity study showed a significant variation in the weight of the mice. The 300 mg/kg dose demonstrated a statistically significant anticonvulsant activity compared to the control group in terms of seizure severity and mortality. It is concluded that the hydroalcoholic root extract of Valeriana pinnatifida R. & P., "valeriana," at a concentration of 300 mg/kg, exhibits an anticonvulsant effect.
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Actividad antidepresiva del extracto etanólico de las hojas de Manihot esculenta Cranz “yuca” en ratones
(Universidad Privada Norbert Wiener, 2023-02-20) Gallardo Delgado, Shirley Jasmin; Justil Guerrero, Hugo Jesús
Depression is a mood disorder characterized by feelings of sadness, with episodes lasting weeks or months, often leading to suicidal thoughts. According to the "WHO," this disease is very common worldwide, affecting more than 280 million people. Over half of those affected do not receive treatment due to a lack of resources or misdiagnosis. Objective: The present study aimed to evaluate the antidepressant activity of the ethanolic extract of Manihot esculenta Cranz leaves ("cassava") in mice. Methodology: The study was experimental and quantitative. The leaves were collected in the district of La Merced, Province of Chanchamayo, Department of Junín. Secondary metabolites of the ethanolic extract were identified. The antidepressant activity (AAD) was evaluated using the forced swimming test in 30 albino mice at doses of 50, 100, and 200 mg/kg of the ethanolic extract administered orally, compared to fluoxetine at 20 mg/kg. Immobility time, swimming time, and climbing time were measured over 15 minutes. Results: The lowest average immobility time was observed in the group treated with the ethanolic extract at 200 mg/kg, with 13.5 seconds, while the control group showed a significantly higher immobility time of 104.2 seconds. The swimming time was highest in the group treated with 200 mg/kg of the ethanolic extract, averaging 177.5 seconds, as was the climbing time, averaging 49.0 seconds. Conclusion: The ethanolic extract at a concentration of 200 mg/kg has antidepressant activity comparable to fluoxetine.
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Actividad antifúngica del extracto de propóleo frente a Candida albicans de pacientes con candidiasis vulvovaginal en el año 2021
(Universidad Privada Norbert Wiener, 2022-06-15) Rodríguez Loyola, Gina Andrea; Collanque Pinto, Jesús Daniel
The general objective of this research was to evaluate the antifungal activity of propolis extract against Candida albicans from patients with vulvovaginal candidiasis in 2021. The research used a quantitative approach with an experimental design. The population consisted of C. albicans strains isolated from patients with vulvovaginal candidiasis admitted to the Gynecology Service of a Private Clinic in Lima. A total of 34 strains were chosen (17 C. albicans strains from acute cases and 8 from recurrent cases) and were treated with propolis extract and fluconazole, with three repetitions, resulting in 102 experimental units. The antifungal susceptibility test was performed using the disk diffusion method with a single concentration of propolis extract and fluconazole as the positive control. Results: The propolis extract, like fluconazole, inhibited all C. albicans strains. However, the effect of fluconazole was significantly greater than that of the research product. Conclusion: The propolis extract does show antifungal activity against C. albicans.
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Actividad antiinflamatoria del extracto etanólico de las hojas del Baccharis buxifolia (Lam.) Pers. “Talla” en ratones
(Universidad Privada Norbert Wiener, 2018-08-22) Rivera Vicuña, David Walter; Chávez Flores, Juana Elvira
The present research aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Baccharis buxifolia (Lam.) Pers. “Talla” leaves in mice. Solubility testing and a qualitative phytochemical profile of the ethanolic extract were carried out. To determine anti-inflammatory activity, the subplantar edema method with 1% albumin, as described by Winter et al. (modified), was employed. Forty-two albino mice (Mus musculus strain Balb/C53) of both sexes, each weighing more than 25 g, were used. The mice were randomly distributed into six groups and subsequently administered 0.1 mL of 1% albumin in the subplantar region. The plant extract was tested at concentrations of 100, 200, and 400 mg/kg of body weight, while dexamethasone (4 mg/kg) and diclofenac (50 mg/kg) were used as standard drugs. The results showed that the ethanolic extract of Baccharis buxifolia leaves, at 400 mg/kg, exhibited the greatest anti-inflammatory effect at two hours, with an inhibition percentage of 62%, compared with the lower extract concentrations (100 mg/kg: 10%; 200 mg/kg: 28%) and with the standard drugs diclofenac (50 mg/kg: 31%) and dexamethasone (4 mg/kg: 48%). It is concluded that the ethanolic extract of Baccharis buxifolia (Lam.) Pers. “Talla” leaves demonstrates significant anti-inflammatory activity at a dose of 400 mg/kg.
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Actividad antimicrobiana del extracto etanólico de las hojas de Schinus molle L. "molle"
(Universidad Privada Norbert Wiener, 2017) Clemente Sotteccani, Claudia Elizabeth; Paucar Lopez, Rosmery; Chávez Flores, Juana Elvira
Peru is one of the countries with the greatest biodiversity in the world, and many of its plant species can be sustainably utilized by industry. Schinus molle L. ("Molle"), a native tree with medicinal applications whose ethanolic extract exhibits antibacterial properties, is a clear example. The objective of this research was to confirm the antimicrobial activity of the ethanolic extract from the leaves of Schinus molle L. To this end, the ethanolic extract was obtained at concentrations of 500 and 1000 mg/mL, with 0.12% chlorhexidine gluconate as the positive control and distilled water as the negative control. The study of antimicrobial activity was conducted using the disk diffusion method with surface plating technique. The experimental solutions were incubated under anaerobic conditions for 48 and 72 hours at 37∘ C, after which the diameters of the inhibition halos were read with a vernier caliper. A qualitative analysis determined the presence of metabolites: flavonoids, alkaloids, carbohydrates, steroids and/or triterpenes, reducing sugars, and phenolic compounds. Results: The ethanolic extract at concentrations of 500 and 1000 mg/mL demonstrated antimicrobial activity against Streptococcus mutans ATCC 25175. From the comparison performed, chlorhexidine gluconate produced greater inhibition. Conclusion: The ethanolic extract of Schinus molle L. 'Molle' presents antimicrobial activity against Streptococcus mutans ATCC 25175, and the chlorhexidine gluconate is qualitatively similar to the ethanolic extract of Schinus molle L. 'Molle'.
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Actividad antioxidante del extracto etanólico de la raíz y el fruto de Euterpe precatoria Mart. "huasaí" procedente de la provincia Maynas y Chanchamayo
(Universidad Privada Norbert Wiener, 2019-11-12) Hernández Cerna, María Elizabeth; Garamendi Alacote, Carmen Rosa; Peña Suasnabar, Carmen Gladys
The process of extracting palm hearts from Euterpe precatoria Mart., 'huasaí,' kills the palm tree. This produces agro-industrial byproducts that should be utilized as an alternative for the root. Objective: To compare the antioxidant activity of the ethanolic extract from the root and fruit of Euterpe precatoria Mart., 'huasaí,' from the province of Maynas, relative to the species native to Chanchamayo. Methodology: The research design was quasi-experimental. The content of total phenols was determined by the Folin-Ciocalteu method, and antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: For antioxidant activity, linear regressions were used to obtain values for the half maximal inhibitory concentration (IC50). The 'huasaí' root from Chanchamayo and Maynas obtained an IC50 of 3.28 µg/mL and 3.86 µg/mL, respectively. The antioxidant activity measurements were contrasted using analysis of variance, Tukey's test (p<0.05), and the Student's t-test. Significant differences were found at the 5% level between the groups from the two provinces and for the three samples. Conclusion: The ethanolic extract from the root, epicarp, and mesocarp of the 'huasaí' fruit from Chanchamayo had greater antioxidant activity than the ethanolic extract from all the Maynas samples.
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Actividad antioxidante del extracto etanólico del mesocarpio del fruto de Hylocereus undatus “pitahaya” e identificación de los fitoconstituyentes
(Universidad Privada Norbert Wiener, 2017-11-08) Figueroa Díaz, Susana Lastenia; Mollinedo Moncada, Ofelia; Félix Véliz, Luis Miguel Visitación
Antioxidants are essential in the human body for preventing oxidative damage, and they can be obtained from different sources such as fruits and plants. The objective of this research was to evaluate the antioxidant activity of the ethanolic extract of the mesocarp of Hylocereus undatus (“pitahaya”) fruit and to determine its phytoconstituents. This quasi-experimental, analytical, descriptive, and prospective study prepared the ethanolic extract and evaluated its antioxidant activity using the DPPH method (2,2-diphenyl-1-picrylhydrazyl, Brand-Williams W). The antioxidant activity was expressed as IC50, with a result of 1.331 μg/mL, indicating significant antioxidant potential. Phytochemical analysis identified carbohydrates, reducing sugars, flavonoids, phenolic compounds, steroids, and alkaloids. In conclusion, the ethanolic extract of the mesocarp of Hylocereus undatus fruit shows antioxidant activity and may be considered a natural source of antioxidants.
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Actividad antioxidante y antiinflamatoria del extracto etanólico de las hojas de Passiflora tripartita var. mollissima (Kunth) "tumbo serrano"
(Universidad Privada Norbert Wiener, 2019-03-08) Ramos Huamaní, Hilda; Pari Colque, Regina; Chávez Flores, Juana Elvira
The plant species Passiflora tripartita var. mollissima (Kunth), commonly known as “tumbo serrano,” is native to Peru and grows in the regions of Cuzco, Lambayeque, Lima, Pasco, Piura, and Talara. The objective of this study was to determine the antioxidant and anti-inflammatory activity of the ethanolic extract of Passiflora tripartita var. mollissima leaves. The ethanolic extract was obtained by maceration, after which solubility testing and phytochemical screening were conducted to identify secondary metabolites. Antioxidant activity was evaluated using the DPPH method, while anti-inflammatory activity was assessed through the paw edema test induced by a phlogistic agent (1% albumin). The results demonstrated antioxidant activity at the following concentrations: 125 µg/mL (79.55%), 93.75 µg/mL (60.99%), 62.5 µg/mL (41.93%), and 31.25 µg/mL (21.82%). Compared with the TROLOX standard, the extract also showed significant anti-inflammatory activity, with a dose of 600 mg/kg producing an effect from the first hour of administration (p = 0.000), which was comparable to that of dexamethasone at 4 mg. In conclusion, the ethanolic extract of Passiflora tripartita var. mollissima leaves exhibits both antioxidant and anti-inflammatory activity.
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Actividad antiulcerosa del extracto etanólico de las hojas de Ficus carica L. "Higo" en ratas
(Universidad Privada Norbert Wiener, 2018-09-17) Abarca Vega, Erika Victoria; Chávez Flores, Juana Elvira
Objective: To determine the antiulcer activity of the ethanolic extract of Ficus carica L. (“Fig”) leaves in rats. An ethanolic maceration of the plant leaves was carried out, followed by solubility testing, preliminary phytochemical analysis, and evaluation of antiulcer activity using the modified Lee (1971) technique at doses of 300, 600, and 800 mg/kg. A total of 60 Holtzman rats were randomly distributed into six groups, induced with gastric ulcer using naproxen 200 mg/kg, with ranitidine 30 mg/kg as the reference drug. The extract was shown to be soluble in methanol, ethanol, and distilled water. The preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, phenolic compounds, and free amino groups. Regarding the antiulcer activity, the most effective treatment was the Ficus carica L. leaf extract at 800 mg/kg, achieving 83.01% inhibition of gastric ulceration, compared with ranitidine, which achieved 73.58% inhibition. These results were obtained using the Marhuenda scale. It was determined that the ethanolic extract of Ficus carica L. leaves at a dose of 800 mg/kg presents antiulcer activity in rats.
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Actividad cicatrizante del extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (paque-paque) en ratones albinos Mus Musculus CEPA BALB/C53
(Universidad Privada Norbert Wiener, 2020-08-13) Heredia Luis, Nuria Lissbeth; Chávez Flores, Juana Elvira
The objective of this research was to confirm the wound-healing activity of the hydroalcoholic extract from the leaves of Ruellia graecizans Backer, 'paque-paque,' in Balb/C53 strain albino mice (Mus musculus). Methodology: The plant was collected in El Rollo, Cutervo, Cajamarca, at an altitude of 1900 m.a.s.l. Solubility tests and a qualitative analysis were performed on the hydroalcoholic extract. A total of 70 mice with an average body weight of 30-40 g were distributed into seven groups. To determine wound-healing activity, gels with the extract at various concentrations (1%, 2%, 5%, and 10%) were prepared and applied to induced lesions using the method described by Vaisberg et al. (1989). These gels were compared to a standard gel and a control base gel. The results showed that the 10% gel had a healing efficacy of over 80%, comparable to the standard gel. Conclusion: The wound-healing activity of the 10% gel based on the topical application of the Ruellia graecizans Backer 'paque-paque' leaf extract was confirmed.
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Actividad cicatrizante del extracto hidroalcohólico de los tallos de Tripogandra serrulata (M. Vahl) Handlos “7 vidas” en ratones albinos
(Universidad Privada Norbert Wiener, 2015) Machuca Cóndor, John José; Chávez Flores, Juana Elvira
This experimental study determined the wound healing effect of the hydroalcoholic extract of Tripogandra serrulata (M. Vahl) Handlos (“7 vidas”) stems in mice. The plant material was collected in the district of Pangoa, province of Satipo, department of Junín, at 628 m a.s.l. The stems were macerated to obtain a homogeneous plant sample. A phytochemical analysis was performed, and topical creams were prepared with different concentrations of the hydroalcoholic extract (5%, 10%, 15%, and 20%), which were used for pharmacological testing according to the method described by Vaisberg et al. (1989), in induced lesions. A total of 56 albino mice (Mus musculus Balb/C53), of both sexes, were distributed into seven groups. The treatment with the greatest efficacy among the prepared topical creams was the 15% concentration. It was concluded that the hydroalcoholic extract of Tripogandra serrulata (M. Vahl) Handlos (“7 vidas”) stems exhibits wound healing activity.
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Actividad cicatrizante y toxicidad dérmica del extracto etanólico de los tubérculos de Ullucus tuberosus Caldas “olluco” en animales de experimentación
(Universidad Privada Norbert Wiener, 2018-05-28) Blacido Paucar, Zayda Judith; Quispe Crisóstomo, Nicols Hilario; Chávez Flores, Juana Elvira
The use of medicinal plants as an alternative to treat ailments and/or diseases is gaining great importance for the creation and research of new formulations. Objective: To determine the wound-healing activity and dermal toxicity of the ethanolic extract from the tubers of Ullucus tuberosus Caldas, 'olluco,' in experimental animals. Methods: The plant species was collected in the Panao community, Huánuco department, Peru. An ethanolic extraction of the Ullucus tuberosus Caldas 'olluco' tuber was performed for phytochemical analysis. The wound-healing activity was assessed using the Vaisberg et al. technique, for which three creams based on the plant species were prepared at concentrations of 0.5,5, and 10%. These were compared with two standard groups: a 1% Croton Lechleri 'Sangre de Drago' cream prepared naturally, and onion plant stem cells (Emolan®), and a control group ('Vaseline'). Dermal toxicity was determined using the Contero technique, where a single oral and dermal dose of 5000 mg/kg of the extract was administered to rats. Results: Phytochemical analysis determined that the extract is soluble in distilled water and contains primary and secondary metabolites. The 10% concentration cream obtained 86% healing efficacy compared to the intact skin sample. In the toxicological evaluation, no Holtzman strain rat showed dermal toxicity. Conclusion: It was determined that the ethanolic extract from the tubers of Ullucus tuberosus Caldas, 'olluco,' does present wound-healing activity and does not possess dermal toxicity.
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Actividad diurética del extracto hidroalcohólico de las hojas secas de Baccharis genistelloides (Lam.) pers. (Carqueja) en ratas albinas de la cepa Holtzman
(Universidad Privada Norbert Wiener, 2021-05-20) López Dávila, Zaid Hebert; Chávez Flores, Juana Elvira
Medicinal plants with diuretic activity currently occupy a place as an alternative therapy to pharmaceutical drugs. Objective: To determine the diuretic activity of the hydroalcoholic extract from the dry leaves of Baccharis genistelloides (Lam.) Pers. (Carqueja) in Holtzman strain albino rats. Materials and method: 50 female rats weighing 230 to 280 g were used, employing the modified Naik et al. model. The rats were pre-hydrated with 0.9% sodium chloride (NaCl) at 50 mL/kg and distributed into five groups: Control Group (0.9% NaCl 50 mL/kg), Standard Group (furosemide 40 mg/kg), Group I (extract 200 mg/kg), Group II (extract 400 mg/kg), and Group III (extract 600 mg/kg). Urine was collected after 6 hours, followed by electrolyte dosage and the calculation of the percentage of urinary volumetric excretion (%EVU) and the percentage of diuretic activity (%AD). Results: At the sixth hour, the highest average urine volume was observed in Group III (8.63 mL). This group also showed 55.7% EVU. Regarding %AD, Group III presented the highest activity at 113.1%, which was superior to the control group. For electrolyte dosage, the observed differences in K+ and Na+ were not significant, meaning it cannot be asserted that the extract significantly alters these electrolytes, while the Cl- values of the extract groups were comparable to the control group. Conclusions: The hydroalcoholic extract from the dry leaves of Baccharis genistelloides (Lam.) Pers. (Carqueja) presented diuretic activity at all three concentrations used.