Examinando por Materia "Platelet Count"

Mostrando 1 - 7 de 7

Acceso abierto
Agreement in the Count and Morphological Identification of Platelets in Blood Smears Between Medical Technologists from Hospitals and Specialized Institutes in Metropolitan Lima and Callao, October 2017 – March 2018
(Universidad Privada Norbert Wiener, 2018-05-31) Conde Sanabria, Ricardo; Rodríguez Ruiz, Lucía Raquel; Alegre Torres, Justo Tobías
The objective is to find the degree of concordance in platelet count and morphological identification on blood smears between Medical Technologists. The study is descriptive, prospective, and cross-sectional. During the sampling period, 106 Medical Technologists working in the Hematology area participated. A 14-question questionnaire, 3 stained blood smears, and 4 platelet morphology images were validated by 3 expert judges. To measure the degree of concordance, Cohen's Kappa coefficient was used. A Kappa coefficient of 0.309, indicating weak agreement for platelet counting on blood smears, and a Kappa of 0.429, indicating moderate agreement for platelet morphological identification, were obtained. To ensure the reliability of the blood smear study, proper sample collection, smear specifications as per CLSI, optimal staining, good review technique, experienced analysts, and quality control programs should be considered. Promoting consensus on hematological cytomorphology is essential to standardize the diverse criteria used today.
Acceso abierto
Análisis comparativo del método automatizado y lectura en lámina para el recuento plaquetario en pacientes del hospital San Bartolomé, 2024
(Universidad Privada Norbert Wiener, 2025-03-21) Atamari Lino, Katherine Elizabeth; Huamán Cárdenas, Víctor Raúl
El recuento plaquetario es una prueba fundamental en el diagnóstico y seguimiento de diversas condiciones médicas, ante una alarma de equipo automatizado se realiza una visualización en lámina periférica para realizar un conteo manual confirmatorio. En distinta lugares como centros de salud o postas, especialmente en áreas rurales o con recursos limitados, los métodos manuales siguen siendo la principal opción debido a la falta de equipos automatizados. Objetivo: Este estudio busca comparar la concordancia analítica entre el método automatizado y las diferentes metodologías de lectura en lámina para el recuento estimado de plaquetas en pacientes del Hospital san Bartolomé, 2024. Materiales y métodos: Se utilizó un diseño de investigación no experimental, transversal y de enfoque cuantitativo. Los datos fueron analizados mediante el coeficiente de correlación de concordancia (CCC) y se aplicaron pruebas estadísticas para determinar la concordancia entre los métodos. El estudio se realizó en el Hospital San Bartolomé, utilizando 131 muestras sanguíneas de pacientes adultos. Se compararon tres métodos de recuento plaquetario: el método automatizado, el método de Fonio y el método del Factor 20 000. Las muestras fueron procesadas en el equipo automatizado de hematología y, posteriormente, se realizaron frotis sanguíneos para los métodos manuales. Los frotis fueron teñidos con Wright Giemsa y evaluados por el encargado del área de hematología. Resultados: Los resultados mostraron una alta concordancia analítica entre el método automatizado y el método de Fonio, con un coeficiente de correlación de concordancia (CCC) de 0.964. En cambio, la concordancia entre el método automatizado y el Factor 20,000 fue de 0.926, indicando una concordancia media. La comparación entre los métodos manuales (Fonio y Factor 20,000) arrojó un CCC de 0.905. Conclusión: El estudio concluye que el método automatizado es el más preciso y confiable para el recuento plaquetario, pero el método de Fonio puede ser una herramienta complementaria, efectiva y también confiable en situaciones donde no se dispone de tecnología avanzada como centros de salud o postas medicas donde la accesibilidad económica y tecnológica es desprotegida.
Acceso abierto
Comparative analysis of the performance of two automated hematological platforms in a level III-I Hospital of the MINSA-Peru in 2023
(Universidad Privada Norbert Wiener, 2024-06-09) Basilio Córdova, Deisy Sumiela; Huamán Cárdenas, Victor Raul
Objective: To compare the analytical performance of the two hematological platforms Beckman Coulter DxH 900 and Mindray BC 6200 used in a level III-I Hospital in Minsa-Peru 2023. Materials and Methods: Based on the CLSI EP15-A3(1) guide, 5 replicates will be performed for each control level over 5 consecutive days, totaling 25 runs per level for leukocytes, erythrocytes, hemoglobin, and platelets. Based on the CLSI EP6-A(2) guide, 5 samples with equidistant concentrations will be tested 3 times for leukocytes and platelets. Results: On the Beckman Coulter DxH900 platform, the Sigma values are 10.9 for leukocytes, 4.6 for erythrocytes, 5.3 for hemoglobin, and 8.1 for platelets. The results for %CVWL(Lab) were lower than the %CVWL(Fab), thus the verification is accepted for each analyte. The overall precision determination for each analyte was based on the limiting level, so the imprecision is 1.5 for leukocytes, 0.8 for erythrocytes, 0.6 for hemoglobin, and 1.6 for platelets. For the Mindray BC6200 platform, the Sigma values are 6.5 for leukocytes, 8.5 for erythrocytes, 7.4 for hemoglobin, and 4.6 for platelets. The %CVWL(Lab) results were lower than the %CVWL(Fab), so the verification is accepted for each analyte. However, the %CVR(Lab) for platelets was rejected at level 1 because it exceeded the %CVR(Fab) and accepted for other levels, but the intralaboratory condition is considered acceptable. The overall precision determination for each analyte was based on the limiting level, with imprecision of 1.6 for leukocytes, 0.7 for erythrocytes, 0.3 for hemoglobin, and 4.8 for platelets. Conclusions: Both platforms met the manufacturer's accuracy and precision specifications, with a favorable Sigma, considering that both platforms worked with the same short quality requirement (VBD). The Beckman platform performed better compared to the Mindray platform.
Acceso abierto
Diagnostic performance of Platelet Indices in the identification of degrees of severity of Gastric Cancer in a hospital center, period 2021 – 2023
(Universidad Privada Norbert Wiener, 2024-04-28) Suarez Meza, Freddy Raul; Huamán Cárdenas, Victor Raul
Stomach cancer is a type of cancer that affects the stomach lining due to the uncontrolled proliferation of its cells, being considered by the WHO as one of the main global health issues. Timely diagnosis, follow-up, and evaluation of people at risk for stomach cancer are essential, and laboratories play a critical role in this process, offering tests for clinicians. The role of platelets in inflammatory processes related to neoplasms is currently being discussed, and it is likely that platelet activation by neoplastic cells contributes to metastasis and cancer progression by stimulating deep vein thrombosis, thrombo-inflammation, and the formation of neutrophil extracellular traps that hide cancer cells. The information provided by platelet analysis is important, as these values can indicate the severity level developed by a person with stomach cancer and potentially improve therapeutic strategies. However, these markers are still under investigation and should be complemented with clinical and epidemiological data to help forecast patient health and provide more personalized treatment.
Acceso abierto
Evaluation of 3 indirect methods for estimated platelet count in peripheral blood slim Lima ‐ Perú, 2022
(Universidad Privada Norbert Wiener, 2023-05-21) Conde Sanabria, Ricardo; Huamán Cárdenas, Victor Raul
The inaccuracy in platelet counts using automated equipment in cases of thrombocytopenia or certain types of thrombocytosis may cause alterations in platelet variability due to the limitations of the methodology. When "Flags" or alerts appear in hematology analyzers, their verification is still carried out using a peripheral blood smear, where qualitative and quantitative alterations in the three blood cell lines can be observed. In Metropolitan Lima and Callao, Clinical Laboratory Technologists who estimate platelet counts in blood smears after detecting platelet alerts in their equipment use various indirect methods based on field parameters or erythrocytic indices, such as the 20,000 factor method, Fonio method, Dameshek method, and others that are less common. Objective: To evaluate the difference between three indirect methods for estimating platelet counts compared to the gold standard in peripheral blood smears. Materials and Methods: The research method is hypothetical-deductive, applied, quantitative, and experimental with a diagnostic study design.
Acceso abierto
Índices plaquetarios y preeclampsia en gestantes atendidas en el centro de salud Baños del Inca, Cajamarca 2023
(Universidad Privada Norbert Wiener, 2024-06-02) Rojas Palomino, Roxana Yudith; Huamán Cárdenas, Víctor Raúl
El estudio es observacional, no experimental y de diseño transversal. La población incluye a 120 gestantes atendidas en el centro de salud Baños del Inca y la muestra será censal, considerando a todas las gestantes que acudan al centro y cumplan con los criterios de inclusión y exclusión. Se utilizará el análisis documentario para la recolección de datos. Los datos serán procesados en Microsoft Excel 2021 y SPSS versión 27. Se crearán tablas de frecuencia y contingencia, se determinará la normalidad de los datos y se emplearán estadígrafos (Pearson o Spearman) según corresponda. Se establecerán puntos de corte para los índices plaquetarios en la detección de preeclampsia, junto con la evaluación de sensibilidad, especificidad y valores predictivos. Se espera encontrar una relación significativa entre los índices plaquetarios y la preeclampsia, lo cual podría permitir una detección temprana de esta condición, contribuyendo a la implementación de medidas preventivas y a mejorar la precisión diagnóstica en entornos con recursos limitados, reduciendo los riesgos asociados a la preeclampsia tanto para las madres como para los fetos.
Acceso abierto
Relationship between mean platelet volume and platelet count with the type of cerebrovascular accident in patients cared for at the National Institute of Neurological Sciences, period 2019 – 2022
(Universidad Privada Norbert Wiener, 2023-11-26) Mendoza Poma, Esmeralda; Huamán Cárdenas, Victor Raul
Stroke is the second leading cause of death worldwide and often results in debilitating sequelae. In our country, its incidence is high, with ischemic cases being predominant and affecting mostly men.