Examinando por Materia "Ratones"

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Actividad analgésica del extracto etanólico de la raíz Vallea stipularis L.F. "Chuillur" en ratones
(Universidad Privada Norbert Wiener, 2018-09-18) Picho Hurtado, Katheryn Elizabeth; Chávez Flores, Juana Elvira
The investigation was carried out on the plant species Vallea stipularis L.f. “Chuillur,” located in the district of Tamburco-Abancay-Apurímac, traditionally used by the local population as medicine for the treatment of scurvy, as a cicatrizant, for gastritis, rheumatism, drastic purgative, and analgesic. Objective: To verify the analgesic activity of the ethanolic extract of the root of Vallea stipularis L.f. “Chuillur” in mice. Methods: The method of abdominal writhing induced by 0.8% acetic acid was employed. Forty-nine albino mice of the species Mus musculus strain Balb/C53/CNPB, of both sexes, were randomly distributed into seven groups. Ethanolic extracts at doses of 50, 100, and 200 mg/kg were used, as well as the standards Paracetamol Q.P. 300 mg/kg and Tramadol Hydrochloride Q.P. 40 mg/kg. These were administered orally, and 30 minutes later, 0.8% acetic acid was administered intraperitoneally. The animals were immediately observed for 20 minutes. Results: All groups showed a significant reduction (p value = 0.000), obtaining differentiated analgesic effects. The maximum effect was achieved with the 200 mg/kg extract, with 70% inhibition, surpassing Paracetamol (55%). Conclusions: It is concluded that the ethanolic extract of the root of the plant species Vallea stipularis L.f. “Chuillur” has analgesic effect.
Acceso abierto
Actividad analgésica y antiinflamatoria del extracto hidroalcólico de hojas y tallos de Leucheria daucifolia (D. Don.) Crisci "Churoq wasin" en ratones
(Universidad Privada Norbert Wiener, 2018-12-07) Soto Requez, Yvon Carla; Ruiz Rondinel, Sandra; Chávez Flores, Juana Elvira
The present study aimed to evaluate the analgesic and anti-inflammatory activity of the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin”. For the analgesic activity, the model of abdominal writhing induced by 0.8% acetic acid was employed; 49 mice were used, and the evaluated groups were: negative control (distilled water), positive control (0.8% acetic acid), hydroalcoholic extract at 50, 100 and 200 mg/kg, compared with paracetamol 300 mg/kg and tramadol 40 mg/kg. For the anti-inflammatory effect, the auricular edema method was used with an irritant agent (6% xylene diluted in acetone) applied to the mouse auricle. A total of 49 albino mice were used, and the study groups were: vaseline cream, creams prepared with the hydroalcoholic extract at 0.5, 1 and 2%, compared with diclofenac 1% cream and hydrocortisone 1% cream. The results show that the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin” at a dose of 100 mg/kg presents analgesic activity with an inhibition percentage of writhing of 62%, not surpassing tramadol (86%) but comparable to paracetamol (76%). Regarding anti-inflammatory activity, the cream with 2% extract presented an inhibition percentage of inflammation of 48.7%, which is comparable to hydrocortisone 1% and diclofenac 1%. It was confirmed that the hydroalcoholic extract possesses both analgesic and anti-inflammatory activity in mice.
Acceso abierto
Actividad ansiolítica y antidepresiva del aceite esencial de las semillas de Foeniculum vulgare Mill. “hinojo” en ratones albinos
(Universidad Privada Norbert Wiener, 2019-10-14) Arotinco Rojas, Karin Griselda; Delgado Cipriano, Clara María de los Angeles; Collantes Llacza, Adela Marlene
According to the World Health Organization (WHO), depression and anxiety account for 4.3% of the global burden of morbidity. The objective of this study was to determine the anxiolytic and antidepressant activity of the essential oil from the seeds of Foeniculum vulgare Mill., 'fennel,' in albino mice. Methodology: The methodology used was experimental, explanatory, quantitative, and applied. The seeds were collected in the province of Huarochirí (Matucana). The essential oil (EO) was extracted by hydrodistillation. The anxiolytic activity was evaluated using the marble burying method in 40 mice at oral doses of 2.5, 5, and 10 mL/kg of EO, compared to diazepam at 10 mg/kg. The antidepressant activity was evaluated using the forced swim test in 40 mice at oral doses of 2.5, 5, and 10 mL/kg of EO, compared to fluoxetine at 20 mg/kg. Results: The essential oil of Foeniculum vulgare showed antidepressant activity at the first two doses and anxiolytic activity at all three doses. Conclusion: The essential oil demonstrated antidepressant activity at doses of 2.5, 5, and 10 mL/kg and showed activity comparable to diazepam.
Acceso abierto
Actividad antiinflamatoria del extracto etanólico de las hojas del Baccharis buxifolia (Lam.) Pers. “Talla” en ratones
(Universidad Privada Norbert Wiener, 2018-08-22) Rivera Vicuña, David Walter; Chávez Flores, Juana Elvira
The present research aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Baccharis buxifolia (Lam.) Pers. “Talla” leaves in mice. Solubility testing and a qualitative phytochemical profile of the ethanolic extract were carried out. To determine anti-inflammatory activity, the subplantar edema method with 1% albumin, as described by Winter et al. (modified), was employed. Forty-two albino mice (Mus musculus strain Balb/C53) of both sexes, each weighing more than 25 g, were used. The mice were randomly distributed into six groups and subsequently administered 0.1 mL of 1% albumin in the subplantar region. The plant extract was tested at concentrations of 100, 200, and 400 mg/kg of body weight, while dexamethasone (4 mg/kg) and diclofenac (50 mg/kg) were used as standard drugs. The results showed that the ethanolic extract of Baccharis buxifolia leaves, at 400 mg/kg, exhibited the greatest anti-inflammatory effect at two hours, with an inhibition percentage of 62%, compared with the lower extract concentrations (100 mg/kg: 10%; 200 mg/kg: 28%) and with the standard drugs diclofenac (50 mg/kg: 31%) and dexamethasone (4 mg/kg: 48%). It is concluded that the ethanolic extract of Baccharis buxifolia (Lam.) Pers. “Talla” leaves demonstrates significant anti-inflammatory activity at a dose of 400 mg/kg.
Acceso abierto
Actividad cicatrizante del extracto hidroalcohólico de las hojas de Ruellia graecizans Backer (paque-paque) en ratones albinos Mus Musculus CEPA BALB/C53
(Universidad Privada Norbert Wiener, 2020-08-13) Heredia Luis, Nuria Lissbeth; Chávez Flores, Juana Elvira
The objective of this research was to confirm the wound-healing activity of the hydroalcoholic extract from the leaves of Ruellia graecizans Backer, 'paque-paque,' in Balb/C53 strain albino mice (Mus musculus). Methodology: The plant was collected in El Rollo, Cutervo, Cajamarca, at an altitude of 1900 m.a.s.l. Solubility tests and a qualitative analysis were performed on the hydroalcoholic extract. A total of 70 mice with an average body weight of 30-40 g were distributed into seven groups. To determine wound-healing activity, gels with the extract at various concentrations (1%, 2%, 5%, and 10%) were prepared and applied to induced lesions using the method described by Vaisberg et al. (1989). These gels were compared to a standard gel and a control base gel. The results showed that the 10% gel had a healing efficacy of over 80%, comparable to the standard gel. Conclusion: The wound-healing activity of the 10% gel based on the topical application of the Ruellia graecizans Backer 'paque-paque' leaf extract was confirmed.
Acceso abierto
Actividad cicatrizante del extracto hidroalcohólico de los tallos de Tripogandra serrulata (M. Vahl) Handlos “7 vidas” en ratones albinos
(Universidad Privada Norbert Wiener, 2015) Machuca Cóndor, John José; Chávez Flores, Juana Elvira
This experimental study determined the wound healing effect of the hydroalcoholic extract of Tripogandra serrulata (M. Vahl) Handlos (“7 vidas”) stems in mice. The plant material was collected in the district of Pangoa, province of Satipo, department of Junín, at 628 m a.s.l. The stems were macerated to obtain a homogeneous plant sample. A phytochemical analysis was performed, and topical creams were prepared with different concentrations of the hydroalcoholic extract (5%, 10%, 15%, and 20%), which were used for pharmacological testing according to the method described by Vaisberg et al. (1989), in induced lesions. A total of 56 albino mice (Mus musculus Balb/C53), of both sexes, were distributed into seven groups. The treatment with the greatest efficacy among the prepared topical creams was the 15% concentration. It was concluded that the hydroalcoholic extract of Tripogandra serrulata (M. Vahl) Handlos (“7 vidas”) stems exhibits wound healing activity.
Acceso abierto
Actividad laxante del extracto hidroalcohólico del fruto Hylocereus undatus (Haw.) Briton & Rose "pitahaya roja" en ratones albinos de la especie Mus musculus
(Universidad Privada Norbert Wiener, 2018-05-14) Berrospi Cristobal, Rosy Katherin; Sánchez Barrera, Mirtha Nancy; Félix Véliz, Luis Miguel Visitación
Today, constipation is a very common problem that can occur at any age. In response, the World Health Organization (WHO) has estimated that more than 80% of the population uses traditional medicine. The sample was collected in the town of Esperanza Alta (La Virgen) in the Huaral district and taxonomically classified at the Natural History Museum of the National University of San Marcos (UNMSM). The purpose of this research was to evaluate the laxative activity of the hydroalcoholic extract from the fruit of Hylocereus undatus (Haw) Britton & Rose, 'red pitahaya,' in albino mice (Mus musculus) using the intestinal transit method. The research was experimental, explanatory, analytical, and prospective. A total of 50 albino mice were used, randomly distributed into seven groups of six animals each. The extracts were administered orally at concentrations of 100, 200, 300, 400, and 600 mg/kg. The positive control group received Bisacodyl (0.25 mg/kg), and the negative control received distilled water. Thirty minutes later, all groups were administered 5% activated charcoal at a dose of 0.1 mL/10 g of body weight. After another 30 minutes, the animals were sacrificed with ether, and a laparotomy-type dissection was performed to measure the distance the charcoal traveled through the small intestine. In conclusion, the laxative activity of the hydroalcoholic extract was confirmed, with an intestinal transit of 74.13% observed at a 400 mg/kg dose. The following phytoconstituents were identified: reducing sugars, saponins, flavonoids, phenolic compounds, alkaloids, fatty compounds, and quinones.
Acceso abierto
Efecto antiinflamatorio y analgésico del extracto etanólico de la cáscara Inga feuillei DC. "pacay" en ratones
(Universidad Privada Norbert Wiener, 2022-02-18) Vásquez Quispe, Ángel David; Villacorta Antón, Santos Enrique; Chávez Flores, Juana Elvira
In Peru, there are various medicinal plants with therapeutic benefits; the plant species Inga feuillei comes from northern Peru (La Libertad - Trujillo). Objective: This study aims to demonstrate the anti-inflammatory and analgesic effect of the ethanolic extract from the peel of Inga feuillei DC, 'pacay,' in mice. Methods: To evaluate the anti-inflammatory effect, 42 rats were divided into 7 groups of 6, and the subplantar edema method described by Winter was used. To evaluate the analgesic effect, 42 mice were divided into 7 groups of 6, and the Koster et al. method was used. Results: The ethanolic extract from the 'pacay' peel showed its greatest analgesic effect at a concentration of 300 mg/kg (87.7%), surpassing the standards of Paracetamol 500 mg/kg (42.8%) and tramadol (86.4%). The greatest anti-inflammatory effect was at a concentration of 500 mg/kg (42.8%), an effect comparable to the standards of Ibuprofen 400 mg/kg and Dexamethasone 4mg/kg. Conclusion: The analgesic and anti-inflammatory effects of the ethanolic extract from the peel of Inga feuillei DC were demonstrated in mice.
Acceso abierto
Efecto hepatoprotector del extracto hidroalcohólico de las hojas de Momordica charantia L. "caigua amarga" en ratones, con intoxicación aguda hepática inducida por paracetamol
(Universidad Privada Norbert Wiener, 2019-04-08) Quispe Toledano, Edwin; Chávez Flores, Juana Elvira
The pharmacological study was conducted with the objective of determining the hepatoprotective effect of the hydroalcoholic extract of the leaves of Momordica charantia L. (“bitter gourd”) in mice with acute liver intoxication induced by paracetamol, and of identifying the different secondary metabolites present in the plant species through the evaluation of the qualitative phytochemical profile. Our design was experimental. For the phytochemical analysis, the leaves of Momordica charantia L. (“bitter gourd”) were collected. Subsequently, the hydroalcoholic extract was obtained by the filtration method. For the pharmacological study, the hydroalcoholic extract of the leaves of Momordica charantia L. was administered at doses of 200, 400, and 600 mg/kg in mice weighing approximately 28–32 g of body weight. The extract was administered orally for three consecutive days, and on the following day, hepatotoxicity was induced with 600 mg/kg of paracetamol. At the end, all animals were subjected to administration of pentobarbital 6.5% 0.1 mL; sacrifice was carried out by the narcosis method, the livers were isolated, and histopathological studies were performed. Results: In the phytochemical screening, the presence of chemical compounds such as alkaloids, phenolic compounds, flavonoids, and tannins was identified. In the histopathological analysis, evidence of cellular damage at the hepatic level was found in all study groups due to the hepatotoxic action of paracetamol. Conclusions: The plant species possesses secondary metabolites with biological activity. The hydroalcoholic extract of the leaves of Momordica charantia L. (“bitter gourd”), at doses of 200, 400, and 600 mg/kg, did not demonstrate hepatoprotective effect in mice with acute paracetamol-induced intoxication.
Acceso abierto
Potencial actividad antiinflamatoria de la crema a partir de las lactonas sesquiterpénicas aisladas de las testas de Persea americana Mill "Palta Fuerte" sobre ratones albinos
(Universidad Privada Norbert Wiener, 2018-07-11) Choquelahua Paquiyauri, Yanina; Illesca Ramón, Jenny; Nué Martinez, Jorge Jonathan
This research evaluated the anti-inflammatory activity of sesquiterpene lactones isolated from the polar fraction of the chloroform extract of Persea americana Mill “Palta fuerte” seeds. For the study, creams were prepared based on the polar fraction of the chloroform extract containing sesquiterpene lactones at different concentrations (1%, 5%, 10%, 20%), reducing inflammation caused by xylene and improving vascular permeability. The results obtained from the xylene-induced inflammation model indicated that creams at 1%, 5%, 10%, and 20% based on the polar fraction of the chloroform extract prevented inflammation of the auricular pavilion (right ear) by 44.94%, 60.20%, 61.04%, and 67.56%, respectively. The anti-inflammatory drugs (Diclofenac 1% gel and Hydrocortisone 1% ointment) induced inflammation inhibition of 64.34% and 81.75%, respectively. These results demonstrate the anti-inflammatory activity of Persea americana Mill “Palta fuerte” seeds.