Examinando por Materia "Staphylococcus aureus"

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Actividad antimicrobiana del aceite esencial de Origanum vulgare L. “orégano peruano” frente a Staphylococcus aureus
(Universidad Privada Norbert Wiener, 2018-05-31) Salinas Segura, Roger; Carhuapoma Yance, Mario
The objective of this research was to confirm the antimicrobial activity of the essential oil of Origanum vulgare L., "Peruvian Oregano," against Staphylococcus aureus. The essential oil was obtained by steam distillation and purified with anhydrous sodium sulfate. The analysis of the main phytochemical components of the oil was performed using organoleptic and instrumental methods. The antimicrobial activity was assayed against Staphylococcus aureus ATCC 6538 using the disk diffusion method (Kirby-Bauer), with Gentamicin 10 µg (OXOID brand) as the positive control and dimethyl sulfoxide as the negative control. Results: The essential oil had a good yield of 2.02%, distilled in 60 minutes, with a density of 0.9132 g/mL and a refractive index of 1.475. The main components were carvacrol and thymol. The essential oil of Origanum vulgare L. showed antimicrobial activity against Staphylococcus aureus ATCC 6538, with inhibition halos of 11.47,13.40,16.60, and 19.65 mm at concentrations of 25,50,75, and 100 µg/mL, respectively. The gentamicin control resulted in an inhibition halo of 30.14 mm. Conclusion: The highest antimicrobial activity of the essential oil of Origanum vulgare L. "Peruvian Oregano" against Staphylococcus aureus was confirmed at 100 µg/mL.
Acceso abierto
Actividad antimicrobiana del extracto etanólico de la raíz de Dracontium spruceanum (Schott) G.H. Zhu “jergón sacha” sobre cepas ATCC de Pseudomonas aeruginosa, Escherichia coli y Staphylococcus aureus, Lima 2024
(Universidad Privada Norbert Wiener, 2025-07-17) Bautista Laura, María del Carmen; Moscoso Mujica, Gladys Angélica
The root of the Dracontium spruceanum species Jergón sacha is found in the tropical forests of Peru and is widely used in traditional medicine. It is frequently used by local residents, and there is little research on its antibacterial activity. Furthermore, there are increasing reports of high resistance to the use of synthetic antimicrobials; therefore, the aim is to provide a natural antimicrobial alternative. The objective of this study was to determine the antimicrobial activity of the ethanolic extract of the root of Dracontium spruceanum (Schott) G.H. Zhu “jergón sacha” on ATCC strains of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus, Lima 2024. In the methodology, the ethanolic extract of the root was obtained and the antimicrobial activity was evaluated at different concentrations of the dry extract by agar diffusion using ATCC strains of Gram positive and Gram negative microorganisms. In the results, the extract in P. aeruginosa showed greater activity than the synthetic antibiotic Cefepime 30 ug with 2.68 mm (p < 0.05); and antimicrobial activity greater than 50% than gentamicin (p < 0.05). In S. aureus it showed antimicrobial activity close to Vancomycin and Oxacillin (p > 0.05); and antimicrobial activity greater than 50% than gentamicin (p < 0.05). In E. coli it showed antimicrobial activity less than 50% of the activity shown by Amoxicillin / clavulanic acid and Sulfamethoxazole / Trimethoprim (p > 0.05), and antimicrobial activity greater than 50% than gentamicin (p < 0.05). It was concluded that the dry ethanolic extract at 100% concentration of the root of Dracontium spruceanum (Schott) G.H. Zhu “jergón sacha” showed antimicrobial activity against ATCC strains of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus, Lima -2024.
Acceso abierto
Comparación de 3 métodos de desinfección en alicates de ortodoncia contaminados con Staphylococcus Aureus
(Universidad Privada Norbert Wiener, 2026-05-11) Yábar Carmona, Johanna Andrea; Pastor Arenas, Sandra Teresa
Esta tesis tuvo como objetivo comparar tres métodos de desinfección en alicates de ortodoncia contaminados experimentalmente con S. aureus. La investigación se planteó como un estudio experimental en el que se evaluó la eficacia de diferentes técnicas de desinfección utilizadas en la práctica odontológica, con el propósito de determinar cuál de ellas ofrecía mayor seguridad y efectividad en la reducción de carga microbiana. Este estudio buscó aportar evidencia que permitiera optimizar los protocolos de bioseguridad en ortodoncia, reduciendo el riesgo de transmisión cruzada de infecciones. Los tres métodos de desinfección (Eucida Advanced, Toallitas desinfectantes marca “FD 333” y Alcohol 70°) eliminaron eficazmente S. aureus en alicates de ortodoncia. Eucida Advanced y Toallitas desinfectantes marca “FD 333” destacaron por su rapidez y practicidad, mientras que el Alcohol 70° fue efectivo, pero requirió mayor tiempo de acción. Los tres métodos fueron viables para la desinfección de alicates contaminados con S. aureus. Sin embargo, Eucida Advanced y Toallitas desinfectantes marca “FD 333” se recomendaron más en la práctica clínica por su mayor eficiencia y menor riesgo de deterioro del instrumental.
Acceso abierto
Efecto antibacteriano del extracto hidroalcohólico de las hojas de solanum aphyodendron s. knapp (olmish) sobre staphylococcus aureus y bacillus cereus in vitro
(Universidad Privada Norbert Wiener, 2024-10-18) Narciso Mauricio, Flor de María; Valdivia Mora, Veronica Maciel; Ñañez del Pino, Daniel
The objective of the research is to determine whether the hydroalcoholic extract of the leaves of Solanum aphyodendron S. Knapp (Olmish) has an antibacterial effect on strains of Staphylococcus aureus ATCC 25923 and Bacillus cereus ATTC 11778, in vitro study. Methodology is hypothetical deductive whose quantitative approach, type of applied research with experimental design, cross-sectional and level of scope is explanatory. The phytochemical analysis was by "Phytochemical screening" and to determine the antibacterial activity was the Kirby Bauer method, which were taken concentrations of 25, 50 and 90 % of the extract. In the results, phenolic compounds, saponins, alkaloids, lactones, tannins, anthocyanins and flavonoids were found. In the microbiological test it was shown that the Staphylococcus aureus strain showed sensitivity to the three concentrations with average inhibition halos of 8,38, 11,10 and 13,42 mm; while Bacillus cereus only showed sensitivity to the concentration of 50 and 90 % with average inhibition halos of 8,63 and 13,02 mm. In conclusion, it was determined that the olmish leaf extract has an antibacterial effect on Staphylococcus aureus at the three concentrations and on Bacillus cereus only at 50 and 90 %, in an in vitro study.
Acceso abierto
Efecto antibacteriano del gel de extracto de Rollinia mucosa (Jacq.) Baill “anona” frente a cepas de colección americana de cultivos
(Universidad Privada Norbert Wiener, 2023-10-12) Fetta More, Karla Solange; Vásquez Carrero, Luz Natali; Collanque Pinto, Jesús Daniel
Research was conducted on the species Rollinia mucosa (Jacq.) Baill “anona,” cultivated in the department of Junín. Objective: To determine the antibacterial effect of the ethanolic extract gel from the leaves of Rollinia mucosa (Jacq.) Baill “anona” against American type culture collection strains. Method: The antibacterial effect was evaluated on strains of Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, and Escherichia coli ATCC 8739 using the Kirby-Bauer method, which measures the diameter of inhibition zones. The strains were cultured on Mueller-Hinton agar plates, and discs impregnated with different concentrations of gels based on the ethanolic extract of the leaves of Rollinia mucosa (Jacq.) Baill “anona” at 0.50%, 0.25%, and 0.15% were applied. The inhibition zones were measured using a measuring instrument. Results: Antibacterial effects were observed at concentrations of 0.50%, 0.25%, and 0.15%, with the highest effect at 0.50% concentration against Staphylococcus aureus (46.67%), Pseudomonas aeruginosa (36.67%), and Escherichia coli (30.67%). Conclusion: The gel based on the ethanolic extract of the leaves of Rollinia mucosa (Jacq.) Baill “anona” demonstrated antibacterial effects at a concentration of 0.50%.
Acceso abierto
Efecto antimicrobiano in vitro del extracto hidroalcohólico de las hojas de Columellia obovata Ruiz & Pav. “pisca pisca” frente a cepas de Staphylococcus aureus ATCC 25923 Y Escherichia coli ATCC 25922. Lima, 2023
(Universidad Privada Norbert Wiener, 2024-10-16) Cerdan Díaz, Percy; Moreto Arraiza, Brian Denzel; Ñañez del Pino, Daniel
The following study aimed to determine the in vitro antimicrobial effect of the hydroalcoholic extract of the leaves of Columellia obovata Ruiz & Pav. “Pisca pisca” against strains of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. In vitro experimental study, cross-sectional, explanatory level, applied type, quantitative approach and hypothetical deductive method. The population was composed of 24 culture media of the ATCC strains. Six groups were used, four experimental groups induced by the extract of Columellia obovata Ruiz & Pav. and controls to gentamicin and physiological saline. To collect data, the agar diffusion method in wells was used, a test that allowed measuring the in vitro susceptibility of the bacteria. The analysis of results was carried out in the SSPS version 25 program, through the Kruskal Wallis statistical test; p<0.05. It was determined that the antimicrobial effect of the hydroalcoholic extract of the leaves of Columellia obovata Ruiz & Pav. “Pisca pisca” at 25% concentrations; fifty%; 75% and 100% showed diameters of inhibition zones in Staphylococcus aureus cultures, with values 11.84 ± 2.51mm; 14.65±1.49mm; 16.75±2.65mm; 18.17 ± 3.48 mm respectively; compared to Gentamicin 34.10 ± 0.79mm. On the other hand, it did not present inhibitory activity against Escherichia coli ATCC 25922 at all concentrations studied and the phytochemical assay identified the presence of phenolic compounds, flavonoids, tannins, anthraquinones, alkaloids and saponins. It is concluded that the hydroalcoholic extract of Columellia obovata leaves demonstrated antimicrobial effect against Staphylococcus aureus ATCC 25923.
Acceso abierto
Evaluación de la efectividad antimicrobiana de un colutorio a base de los aceites esenciales de Eucalyptus globulus Labill (eucalipto) y Minthostachys sp. (muña), frente a Klebsiella pneumoniae ATCC 10031, Staphylococcus aureus ATCC 29737 y Candida albicans ATCC 10231
(Universidad Privada Norbert Wiener, 2017-12-06) Aylas Canicela, Roosevelt Edhair; Nué Martinez, Jorge Jonathan
The present research work entitled “Evaluation of the antimicrobial effectiveness of a mouthwash based on the essential oils of Eucalyptus globulus Labill (Eucalyptus) and Minthostachys sp. (Muña), against Klebsiella pneumoniae ATCC 10031, Staphylococcus aureus ATCC 29737 and Candida albicans ATCC 10231”, had as its main objective to evaluate the antimicrobial effectiveness of the formulated mouthwash. The study was quasi-experimental, prospective, and cross-sectional. A registration form was used for the data obtained. The method employed for the evaluation of the antibacterial effectiveness was the Modified Microbial Challenge test. The results were: for Klebsiella pneumoniae, at a significance level of 0.05 and a p-value = 0.4412, the treatment with the formulated mouthwash has the same effect as the commercial mouthwash; for Staphylococcus aureus, at a significance level of 0.05 and a p-value = 0.6039, the treatment with the formulated mouthwash is not more effective than the commercial mouthwash, both present the same effectiveness; for Candida albicans, at a significance level of 0.05 and a p-value = 0.0000325, it is accepted that the treatment with the formulated mouthwash shows greater effectiveness than the commercial mouthwash.
Acceso abierto
Factores personales-laborales de estudiantes asintomáticos y su relación con la presencia de staphylococcus aureus meticilino resistente E.A.P. Tecnología Médica Universidad Privada Norbert Wiener. Lima – 2015
(Universidad Privada Norbert Wiener, 1905-07-07) Castillo Paiva, Elmer; Benites Azabache, Juan Carlos
Staphylococcus aureus es una bacteria Gram positiva capaz de convertirse en un patógeno de difícil manejo, y es uno de los principales causantes de infecciones a nivel comunitario y hospitalario, A lo largo de la historia se ha evidenciado que diferentes bacterias generan mecanismos de resistencia a múltiples antimicrobianos, lo cual dificulta el manejo terapéutico. La resistencia antimicrobiana se debe, entre otras causas, al uso indiscriminado de antibióticos, al mal manejo por parte de los profesionales de la salud, a la venta sin ningún tipo de control de los medicamentos y a la automedicación pues hace que se aumenten las probabilidades que los microorganismos muten y/o adquieran genes de resistencia por ciclos incompletos de administración prolongando así la duración de la enfermedad y, a su vez, aumenta el riesgo de muerte. El trabajo busca determinar los Factores personales-laborales de estudiantes asintomáticos y su relación con la presencia de Staphylococcus aureus meticilino resistentes. E.A.P. Tecnología Médica. Universidad Privada Norbert Wiener. Lima – 2015. La población estuvo conformada por 50 alumnos que pertenecen a la comunidad universitaria de la Universidad Norbert Wiener, de las cepas de Staphylococcus aureus aisladas en 06 estudiantes ninguna fue identificada como SAMR.
Acceso abierto
Relación entre la formación de biofilm y resistencia a oxacilina en staphylococcus aureus aislados de hemocultivos. Hospital Nacional Hipólito Unanue, julio 2016 – junio 2018
(Universidad Privada Norbert Wiener, 2020-01-18) Zavala Castillo, Rosa Iris; Yalle Cotrina, Oliver Francisco; Champi Merino, Roky Govanni
La presente investigación tuvo como objetivo, determinar la relación entre la formación de biofilm y resistencia a oxacilina en Staphylococcus aureus aislados de hemocultivos en el Hospital Nacional Hipólito Unanue. La muestra estuvo conformada por 71 cepas de Staphylococcus aureus aislados de hemocultivos. El estudio fue de tipo cuantitativo, observacional, correlacional. Para la evaluación de los datos se utilizó los programas estadísticos SPSS versión 22 y WHONET 5.6, siendo empleado para la asociación de variables la prueba estadística Chi cuadrado. Y para la determinación de la frecuencia de Staphylococcus aureus resistente a oxacilina, la tabla de puntos de corte según los criterios del Instituto de Laboratorio Clínico (CLSI) encontrándose un 63.4% de resistencia a oxacilina en Staphylococcus aureus. Así mismo, se utilizó el método en placa de cultivo de tejido (PCT) para la determinación de biofilm, fueron distribuidas según su capacidad de formación, se observó 55 cepas (77.5%) con la capacidad de formación de biofilm y 16 cepas (22.5%) fueron no formadoras de biofilm. Del mismo modo se estratificó las cepas de Staphylococcus aureus aislado de hemocultivos por su capacidad de formación de biofilm, fuerte 49.3%, formación moderada 18.3%, y con formación débil 9.9%. El 22.5% de cepas no presento capacidad de formar biofilm. De la investigación se concluyó que no existe relación significativa entre la resistencia a la oxacilina y la formación de biofilm en Staphylococcus aureus aislado de hemocultivos de pacientes hospitalizados. Hospital Nacional Hipólito Unanue, Julio 2016 – junio 2018.
Acceso abierto
Resistencia MLSB del Staphylococcus aureus en bacteriemias en el Hospital Nacional Edgardo Rebagliati Martins, 2019
(Universidad Privada Norbert Wiener, 2021-12-09) Saravia Tasayco, David; Gonzales Rojas, Víctor Hugo; Champi Merino, Roky Govanni
El Staphylococcus aureus es un patógeno intrahospitalario por excelencia que causa bacteriemias y en muchos casos son mortales. Se estudió 154 cepas aisladas de hemocultivos de pacientes, para la identificación bacteriana y prueba de susceptibilidad antim