Actividad analgésica y antiinflamatoria del extracto hidroalcólico de hojas y tallos de Leucheria daucifolia (D. Don.) Crisci "Churoq wasin" en ratones

Abstract

The present study aimed to evaluate the analgesic and anti-inflammatory activity of the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin”. For the analgesic activity, the model of abdominal writhing induced by 0.8% acetic acid was employed; 49 mice were used, and the evaluated groups were: negative control (distilled water), positive control (0.8% acetic acid), hydroalcoholic extract at 50, 100 and 200 mg/kg, compared with paracetamol 300 mg/kg and tramadol 40 mg/kg. For the anti-inflammatory effect, the auricular edema method was used with an irritant agent (6% xylene diluted in acetone) applied to the mouse auricle. A total of 49 albino mice were used, and the study groups were: vaseline cream, creams prepared with the hydroalcoholic extract at 0.5, 1 and 2%, compared with diclofenac 1% cream and hydrocortisone 1% cream. The results show that the hydroalcoholic extract of leaves and stems of Leucheria daucifolia (D. Don.) Crisci “Churoq wasin” at a dose of 100 mg/kg presents analgesic activity with an inhibition percentage of writhing of 62%, not surpassing tramadol (86%) but comparable to paracetamol (76%). Regarding anti-inflammatory activity, the cream with 2% extract presented an inhibition percentage of inflammation of 48.7%, which is comparable to hydrocortisone 1% and diclofenac 1%. It was confirmed that the hydroalcoholic extract possesses both analgesic and anti-inflammatory activity in mice.

El presente estudio tuvo como objetivo evaluar la actividad analgésica y antiinflamatoria del extracto hidroalcohólico de hojas y tallos de Leucheria daucifolia(D. Don.) Crisci “Churoq wasin”. Para la actividad analgésica se empleó el modelo de contorsiones abdominales por ácido acético al 0,8%, se utilizaron 49 ratones y los grupos evaluados fueron: Control negativo (agua destilada), control positivo (ácido acético al 0,8%), extracto hidroalcohólico 50, 100 y 200 mg/kg y se comparó con paracetamol 300 mg/kg y tramadol 40 mg/kg. Para el efecto antiinflamatorio se empleó el método de edema auricular por un agente irritante xilol al 6% diluido en acetona en el pabellón auricular del ratón. Se usaron 49 ratones albinos y los grupos de estudios fueron: crema vaselina,crema elaboradas a base del extracto hidroalcohólico al 0,5, 1 y 2%, comparando con el diclofenaco 1% crema e hidrocortisona al 1% crema. Los resultados evidencian que el extracto hidroalcohólico de hojas y tallos de Leucheria daucifolia(D. Don.) Crisci “Churoq wasin”a dosis 100 mg/kg presenta actividad analgésica con un porcentaje de inhibición de contorsiones del (62%) no superando al tramadol (86%) y es comparable al paracetamol (76%); y con respecto a la actividad antinflamatoria, la crema del extracto al 2% presenta un porcentaje de inhibición de la inflamacióncon un (48.7%) el cual es comparable a la hidrocortisona 1%y el diclofenaco 1%. Se comprobó que existe actividad analgésica y actividad antiinflamatoria en ratones.