Actividad antimicrobiana in vitro de pastas medicadas utilizadas en tratamiento pulpar odontopediátrico, frente al Enterococos Faecalis, Lima 2025

Abstract

El objetivo fue comparar la actividad antimicrobiana in vitro de pastas medicadas empleadas en tratamientos pulpares odontopediátricos frente a Enterococcus faecalis. Se desarrolló un estudio cuantitativo, básico y experimental (corte longitudinal, nivel explicativo), utilizando la cepa de referencia E. faecalis ATCC 29212. El inóculo se ajustó a 0.5 McFarland y se sembró en agar Mueller-Hinton; se aplicaron las pastas TAP, 3Mix, CTZ y Guedes Pinto mediante pozos/discos, con incubación a 37 °C y medición de halos inhibitorios (mm) a 24, 72 y 168 horas. Los datos fueron sometidos a Shapiro–Wilk y análisis inferencial con ANOVA (p<0.05). Los resultados mostraron diferencias significativas globales entre pastas (ANOVA, p=0.000). CTZ presentó la mayor actividad promedio (media 42.648 mm), seguida de 3Mix (35.3313 mm) y Hoshino (33.5553 mm); Guedes Pinto evidenció la menor actividad (19.9763 mm). En el análisis temporal, solo TAP mostró variación significativa entre 24, 72 y 168 horas (p=0.000), mientras que 3Mix, CTZ y Guedes Pinto no evidenciaron cambios significativos (p>0.05). Se concluye que CTZ fue la pasta más efectiva frente a E. faecalis en las condiciones evaluadas.

The objective was to compare the in vitro antimicrobial activity of medicated pastes used in pediatric dental pulp treatments against Enterococcus faecalis. A quantitative, basic, and experimental study (longitudinal design, explanatory level) was conducted using the reference strain E. faecalis ATCC 29212. The inoculum was adjusted to 0.5 McFarland and plated on Mueller-Hinton agar; the TAP, 3Mix, CTZ, and Guedes Pinto pastes were applied using wells/discs, incubated at 37 °C, and inhibitory zones (mm) were measured at 24, 72, and 168 hours. The data were subjected to Shapiro-Wilk and inferential analysis using ANOVA (p<0.05). The results showed significant overall differences between toothpastes (ANOVA, p=0.000). CTZ exhibited the highest average activity (mean 42.648 mm), followed by 3Mix (35.3313 mm) and TAP (33.5553 mm); Guedes Pinto showed the lowest activity (19.9763 mm). In the temporal analysis, only TAP showed significant variation between 24, 72, and 168 hours (p=0.000), while 3Mix, CTZ, and Guedes Pinto did not show significant changes (p>0.05). It is concluded that CTZ was the most effective toothpaste against E. faecalis under the evaluated conditions.