Antibacterial action of irrigating compounds using 5% sodium hypochlorite, 17% ethylenediaminetetraacetic acid and 2% chlorhexidine gluconate, against enterococcus faecalis culture strains through an in vitro study Lima-2022

Abstract

The main objective of this study was to evaluate the antibacterial action of the irrigant compounds Sodium Hypochlorite at 5%, Ethylenediaminetetraacetic Acid (EDTA) at 17%, and Chlorhexidine Gluconate at 2% against Enterococcus faecalis strains. This in vitro experimental study, cross-sectional, and prospective, included root canal irrigants and 15 wells per group in Petri plates inoculated with Enterococcus faecalis. To assess the antibacterial action, the agar diffusion method was used, and the samples were incubated at 37°C. After 24 hours, the bacterial inhibition zones were measured and recorded. Kruskal-Wallis test was used for statistical analysis. The results showed that EDTA at 17% had the highest antibacterial action against E. faecalis, presenting an inhibition zone of 25 mm, followed by Chlorhexidine Gluconate at 2% with an inhibition zone of 23 mm, and Sodium Hypochlorite at 5% with an inhibition zone of 11 mm. The study concluded that EDTA at 17% has significantly higher antibacterial action compared to Chlorhexidine Gluconate at 2%, followed by Sodium Hypochlorite at 5% against Enterococcus faecalis.

El objeto fundamental de este estudio es evaluar la acción antibacteriana de los compuestos irrigantes Hipoclorito de sodio al 5%, ácido etilendiaminotetraacético al 17% y gluconato de clorhexidina al 2% frente a cepas de cultivo de Enterococcus faecalis. Este estudio experimental in vitro, transversal, prospectivo, incluyó a los irrigantes del conducto radicular y 15 pocillos por grupo en placas Petri inoculadas con Enterococcus faecalis, para hallar la acción antibacteriana se dispuso del método de difusión en agar, las muestras se incubaron a 37°C y fueron retiradas para medir y registrar las zonas de inhibición bacteriana al cabo de 24 horas. Para el análisis estadístico de los datos conseguidos se usó la prueba de Kruskal-Wallis. Como resultados se obtuvo que el EDTA al 17% tuvo mayor acción antibacteriana frente a E. faecalis presentando zona de inhibición de 25 mm, gluconato de clorhexidina al 2% con halo de inhibición de 23mm y el hipoclorito de Sodio al 5% con halo de inhibición de 11mm. Se concluyó que EDTA al 17% posee una acción antibacteriana considerablemente mayor que el gluconato de clorhexidina al 2% seguido por el hipoclorito de sodio al 5% frente al Enterococcus faecalis.