Antibacterial effect of ethanolic extract of propolis at 30% and cetylpyridinium chloride at 0.05% plus chlorhexidine digluconate at 0.05% on strains of Streptococcus mutans (ATCC 25175). In Vitro comparative study. 2021.

Abstract

This is an in vitro experimental study using two samples: Sample 1, 30% ethanol propolis extract, and Sample 2, cetylpyridinium chloride at 0.05% plus chlorhexidine digluconate at 0.05%, with chlorhexidine digluconate at 0.12% as the control group. Inhibition halos were measured around each of the discs soaked with the antibacterial substances placed on the plates. The plates were incubated for 48 hours, and the halos were measured using a Vernier caliper. The Duraffourd et al. scale (1986) was used for interpreting the antibacterial effect against Streptococcus mutans. The streaking and exhaustion technique was used on the agar. A total of 30 experimental units were plated on blood agar, on which 3 sterile antibiogram discs of 6mm each were placed, inoculated with Streptococcus mutans.

De tipo experimental in vitro, usando como muestra 1, el extracto etanólico de propóleo al 30% y muestra 2, cloruro de cetilpiridinio al 0.05% mas digluconato de clorhexidina al 0.05% y como grupo control el digluconato de clorhexidina al 0.12%. Se realizó la medición de halos de inhibición que se formaron alrededor de cada uno de los discos embebidos de las sustancias antibacterianas colocados en las placas, se incubarán por 48 horas y se medirán con regla de vernier. Se usará la escala de Duraffourd et al., 1986 para la interpretación del efecto antibacteriano frente a streptococcus mutans. Se usará la técnica de estriado y agotamiento en los agares. Se sembraron 30 unidades experimentales en agar sangre en el cual se colocarán 3 discos estériles de antibiograma de 6mm por placa inoculadas con Streptococcus Mutans.