Examinando por Autor "Malpartida Quispe, Federico Martin"

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(Universidad Privada Norbert Wiener, 2017-12-18) Huallpa Tucto, Elizabeth Evelyn; Malpartida Quispe, Federico Martin
The objective of this study was to evaluate the inhibitory effect of the essential oil of muña mixed with calcium hydroxide, compared to four solutions, against Enterococcus faecalis (ATCC 29212). The agar well diffusion method with 6 mm diameter wells was applied to examine the substances, including saline solution (negative control). The inhibition zones were measured at 24 and 72 hours, with the following results: 16.17 mm and 15.36 mm for the essential oil of muña mixed with calcium hydroxide; 9.18 mm and 8.68 mm for the essential oil of muña; 19.89 mm and 17.09 mm for the 3Mix-MP paste; 21.40 mm (for both time periods) for 2% chlorhexidine gluconate; and 17.89 mm and 15.60 mm for the mixture of 2% chlorhexidine gluconate with calcium hydroxide. The study concluded that the inhibitory effect of the essential oil of muña mixed with calcium hydroxide was lower than that of the 3Mix-MP paste, the 2% chlorhexidine gluconate, and the mixture of 2% chlorhexidine gluconate with calcium hydroxide, but greater than that of the essential oil of muña.
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(Universidad Privada Norbert Wiener, 2015) Carrión Reyes, Gueraldin Mercedes; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the ethanolic extract of Bidens pilosa (Amor seco) compared to Colgate Plax® and 0.12% Chlorhexidine Gluconate against strains of Streptococcus mutans. The study was experimental, prospective, longitudinal, and analytical. The population consisted of Streptococcus mutans strains (ATCC 25175), and the sample consisted of 40 Petri dishes. The strains were reactivated and cultured on Blood Agar, where 6 mm diameter wells were made, and approximately 100 µl of the ethanolic extract of Bidens pilosa (Amor seco) were added in the following concentrations: 0.8/10, 1/10, and pure. The results were compared with 0.12% Chlorhexidine Gluconate as a positive control, distilled water as a negative control, and Colgate Plax® mouthwash. The results showed that the 0.8/10 and 1/10 concentrations of the ethanolic extract of Bidens pilosa (Amor seco) did not present any inhibition zone at any time. However, the pure ethanolic extract produced an average inhibition zone of 11.98 mm at 24 hours and 11.80 mm at 48 hours. Furthermore, a stronger inhibitory effect was observed with the positive control, 0.12% Chlorhexidine Gluconate, and a lesser inhibitory effect compared to Colgate Plax® mouthwash against the Streptococcus mutans (ATCC 25175) strain.
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(Universidad Privada Norbert Wiener, 2015) Juscamaita Gutierrez, Elizabeth Stefany; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the essential oil of Eucalyptus globulus compared to 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash on the bacterial inhibition of Streptococcus mutans. The study was experimental, cross-sectional, prospective, and analytical, with an explanatory level, conducted in a laboratory. The population consisted of Streptococcus mutans (ATCC 25175) strains, and the sample included 40 Petri dishes. The culture medium used was Blood Agar, and 6 mm diameter wells were made in each Petri dish using a sterile punch. The essential oil of Eucalyptus globulus at concentrations of 25%, 50%, and 100%, 0.12% Chlorhexidine Gluconate, Colgate Plax® mouthwash, and Tween 20 were added to the wells. The results showed that the 25%, 50%, and 100% concentrations of Eucalyptus globulus essential oil produced inhibition halos with average diameters of 6.88 mm, 9.61 mm, and 12.65 mm at 24 hours, and 6.88 mm, 9.53 mm, and 11.90 mm at 48 hours, respectively, which were smaller than the inhibition halos produced by 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash at both 24 and 48 hours against the Streptococcus mutans (ATCC 25175) strain.
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(Universidad Privada Norbert Wiener, 2017-11-09) Ponce Retamoso, Jesús Antonio; Malpartida Quispe, Federico Martin
Oral health pathology is a public health problem among the population in our country, with a high incidence primarily related to dental caries. The objective of this study was to determine the level of knowledge about oral hygiene habits in secondary school students at the Colegio Nacional Virgen del Socorro in Pomabamba, Ancash, 2017. The study sample consisted of 291 secondary school students of both sexes. A non-probabilistic, convenience sampling technique was used, based on inclusion and exclusion criteria. A validated 12-question closed-ended survey with multiple-choice options regarding oral hygiene habits was developed. The results showed that the level of knowledge about oral hygiene habits among the students of both sexes was poor in 34%, regular in 48.4%, and good in 17.6%.
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(Universidad Privada Norbert Wiener, 2015) Cerga Rodriguez, Leslie Alexandra; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of essential oils from Cinnamomum zeylanicum (cinnamon) and Syzygium aromaticum (clove) in comparison to 2% Chlorhexidine Gluconate against Enterococcus faecalis strains. The study was experimental, prospective, longitudinal, and analytical. The essential oils from these plants were obtained using the steam distillation method, utilizing the bark of Cinnamomum zeylanicum (cinnamon) and dried buds of Syzygium aromaticum (clove) at concentrations of 1%, 5%, 25%, 50%, 75%, and 100%. For the experiment, Enterococcus faecalis (ATCC 29212) was reactivated and then cultured on Petri dishes (15 cm in diameter) containing Mueller Hinton agar with 6 mm diameter wells, where approximately 100 µl of the essential oils were added, compared to 2% Chlorhexidine Gluconate as a positive control and Tween 20 as a negative control. The plates were incubated at 37°C to check for bacterial viability and media sterility, and the inhibition zones were measured using a Vernier caliper at 24 and 48 hours. In vitro sensitivity testing yielded the following results: the antimicrobial effect of Cinnamomum zeylanicum (cinnamon) essential oil at its minimum effective concentration (1%) was greater than the antimicrobial effect of Syzygium aromaticum (clove) essential oil at its minimum effective concentration (25%). Additionally, a greater antimicrobial effect was observed with the positive control, 2% Chlorhexidine Gluconate.
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(Universidad Privada Norbert Wiener, 2015) Carhuaricra Soto, Yudiht Abigail; Malpartida Quispe, Federico Martin
In periodontal disease, various microorganisms are involved, one of which is Porphyromonas gingivalis, an obligate anaerobic, Gram-negative bacterium that is predominantly found in chronic periodontitis, and is commonly eliminated by 0.12% chlorhexidine. The purpose of this study was to determine the inhibitory effect of Rosmarinus officinalis (rosemary) essential oil and 0.12% chlorhexidine on Porphyromonas gingivalis, using the agar diffusion method with discs, as an effort to find an alternative, natural, effective, and economical antimicrobial substance against this bacterium. For this, Porphyromonas gingivalis strains were inoculated in 40 Petri dishes containing Mueller Hinton agar. Then, three discs were placed on each plate, impregnated with 20 µl of 0.12% chlorhexidine, Rosmarinus officinalis essential oil, and distilled water as a negative control. The plates were incubated at 37°C in an anaerobic jar for the duration of the study, being removed only to measure the inhibition halos generated at 24 and 48 hours. The data were processed using SPSS software, and the Student's T-test was applied. It was concluded that Rosmarinus officinalis (rosemary) essential oil exhibited an inhibitory effect against Porphyromonas gingivalis, and 0.12% chlorhexidine had a greater inhibitory effect than the essential oil against this bacterial strain.
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(Universidad Privada Norbert Wiener, 2015) Carrión Reyes, Fiorella Isamar; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the essential oil of Schinus molle (Molle) compared to 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash in inhibiting bacterial growth of Streptococcus mutans in vitro. The study was experimental, cross-sectional, prospective, and analytical at an explanatory level, conducted in a microbiology laboratory. The population consisted of Streptococcus mutans strains (ATCC 25175), with a sample of 40 Petri dishes. The agar diffusion method was used with 6 mm diameter wells, where 100 µl of Schinus molle (Molle) essential oil at 25%, 50%, and 100%, 0.12% Chlorhexidine Gluconate, Colgate Plax® mouthwash, and Tween 20 were poured. The results showed that the concentrations of 50% and 100% Schinus molle essential oil produced an average inhibition zone of 10.97 mm and 10.46 mm at 24 hours, and 11.06 mm and 10.59 mm at 48 hours, which were smaller than the inhibition zones formed by 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash at both times against the Streptococcus mutans (ATCC 25175) strain.
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(Universidad Privada Norbert Wiener, 2015) Rojas Herrera, Jose Antonio; Malpartida Quispe, Federico Martin
The objective of this study was to determine the relationship between the level of knowledge and behavior regarding oral hygiene in 118 secondary school students aged 11 to 19 years from the Institución Educativa Integrado Agropecuaria La Florida, located in the La Florida-Perené-Chanchamayo populated center in 2015. The modified HU-DBI (Hiroshima University–Dental Behavioral Inventory) questionnaire on oral hygiene behavior and knowledge was applied, which was validated and translated into Spanish. The data were organized into tables and graphs, using descriptive statistics and the Chi-square test to establish the relationship between knowledge level and behavior. The study concluded that the level of knowledge and behavior regarding oral hygiene was regular across both genders and all ages. Furthermore, there was a direct relationship between the level of knowledge and behavior regarding oral hygiene among these students.
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(Universidad Privada Norbert Wiener, 2014) Zavala Vega, Luis Antonio; Malpartida Quispe, Federico Martin
The presence of bacteria in the root canal induces chronic periapical disease. Therefore, one of the most important objectives of endodontic treatment is to eliminate all microorganisms from the root canals, using both irrigating solutions and intra-canal medication between sessions, especially in cases of endodontic retreatment, due to the presence of persistent bacteria that may resist various commonly used intra-canal medications. Enterococcus faecalis are the most frequently isolated bacteria from teeth with endodontic failure and the main contributors to persistent apical periodontitis. The objective of this study was to evaluate the inhibitory effect of 2% chlorhexidine gel and calcium hydroxide mixed with various vehicles (2% chlorhexidine solution, camphorated paramonochlorophenol, and saline solution) against Enterococcus faecalis, using the agar well diffusion method. Fifty Petri dishes with brain heart agar were prepared, with each dish containing 4 wells saturated with intra-canal medications. The samples were incubated at 37°C, and measurements of bacterial inhibition zones were recorded at 1, 7, and 15 days. The data were processed using the analysis of variance statistical test, concluding that 2% chlorhexidine gel has greater antibacterial efficacy than calcium hydroxide combined with various vehicles (2% chlorhexidine solution, camphorated paramonochlorophenol, and saline solution) after 1, 7, and 15 days against Enterococcus faecalis.
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Antibacterial and Antiadhesion Effects of Psidium guajava Fractions on a Multispecies Biofilm Associated with Periodontitis
(Association of Support to Oral Health Research (APESB), 2022) Millones Gómez, Pablo Alejandro; Requena Mendizábal, Margarita Fe; Calla Poma, Roger Damaso; Rosales Cifuentes, Tania Valentina; Malpartida Quispe, Federico Martin; Maurtua Torres, Dora Jesús; Bacilio Amaranto, Reyma Evelyn; Minchón Medina, Carlos Alberto; Ponce Contreras, Lusin Antonio
Objective: To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material and Methods: Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test. Results: The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii(p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction. Conclusion: The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.
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Antibacterial and Antiadhesion Effects of Psidium guajavaFractions on aMultispecies Biofilm Associated with Periodontitis
(Association of Support to Oral Health Research (APESB), 2022-05-16) Millones Gómez, Pablo Alejandro; Requena Mendizábal, Margarita Fe; Calla Poma, Roger Damaso; Rosales Cifuentes, Tania Valentina; Malpartida Quispe, Federico Martin; Maurtua Torres, Dora Jesús; Bacilio Amaranto, Reyma Evelyn; Minchón Medina, Carlos Alberto; Ponce Contreras, Lusin Antonio
Objective:To assess the antibacterial activity of Psidium guajavafractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalisin vitro. Material and Methods:Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajavawas evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobialsusceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test.Results:The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii(p=0.000), F. nucleatum(p=0.000), and P. gingivalis(p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction. Conclusion:The methanol fraction of P. guajavahad an antibacterial effect on S. gordoniiand P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.
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EFECTO INHIBIDOR DE LAS CONCENTRACIONES MÍNIMAS DEL ACEITE ESENCIAL DE MUÑA Y ORÉGANO EN COMPARACIÓN CON SUSTANCIAS ANTIMICROBIANAS USADAS EN LA TERAPIA PULPAR FRENTE A CEPAS DE Enterococcus faecalis. ESTUDIO IN VITRO. LIMA 2014.
(Universidad Privada Norbert Wiener, 2017-02-28) Malpartida Quispe, Federico Martin; Aguirre Morales, Anita Kori
El objetivo del presente estudio fue determinar el efecto inhibidor de las concentraciones mínimas del aceite esencial de Minthostachys mollis (muña) y Origanum vulgare (orégano) en comparación con sustancias antimicrobianas usadas en la terapia pulpar frente a cepas de Enterococcus faecalis. Para tal efecto se reactivaron los E. faecalis (ATCC 29212) que fueron utilizados en las dos etapas del estudio: 1.- Determinación de la concentración mínima inhibitoria (CMI) de ambos aceites esenciales. 2.- Determinación del efecto inhibidor de estos aceites esenciales en comparación a sustancias antimicrobianas usadas en la terapia pulpar. En la primera etapa del estudio se encontró que la CMI del aceite esencial de muña fue 10% y aceite esencial de orégano fue 30%. En la segunda etapa, los E. faecalis fueron sembrados en 56 placas petri que contenían el medio de cultivo Mueller Hinton con pozos de 6 mm. de diámetro donde se vertieron 100 ul. de aceite esencial de muña al 100%, aceite esencial de muña al 10% (CMI), aceite esencial de orégano al 100%, aceite esencial de orégano al 30% (CMI), hipoclorito de sodio al 5%, clorhexidina al 2% (gel), clorhexidina al 2% (solución), Calcifar - P ® y Tween 20. Las placas se incubaron a 37°C realizándose la medición de los halos de inhibición con un calibrador vernier o regla pie de rey a las 24 y 48 horas. Para el análisis de los resultados se utilizaron las pruebas de ANOVA y Tukey. Se concluye que la CMI del aceite esencial de muña y orégano tienen efecto inhibidor frente a E. faecalis a las 24 y 48 horas, sin embargo su efecto es menor que la Clorhexidina al 2% (solución).