Examinando por Materia "Enterococcus faecalis"

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(Universidad Privada Norbert Wiener, 2017-12-18) Huallpa Tucto, Elizabeth Evelyn; Malpartida Quispe, Federico Martin
The objective of this study was to evaluate the inhibitory effect of the essential oil of muña mixed with calcium hydroxide, compared to four solutions, against Enterococcus faecalis (ATCC 29212). The agar well diffusion method with 6 mm diameter wells was applied to examine the substances, including saline solution (negative control). The inhibition zones were measured at 24 and 72 hours, with the following results: 16.17 mm and 15.36 mm for the essential oil of muña mixed with calcium hydroxide; 9.18 mm and 8.68 mm for the essential oil of muña; 19.89 mm and 17.09 mm for the 3Mix-MP paste; 21.40 mm (for both time periods) for 2% chlorhexidine gluconate; and 17.89 mm and 15.60 mm for the mixture of 2% chlorhexidine gluconate with calcium hydroxide. The study concluded that the inhibitory effect of the essential oil of muña mixed with calcium hydroxide was lower than that of the 3Mix-MP paste, the 2% chlorhexidine gluconate, and the mixture of 2% chlorhexidine gluconate with calcium hydroxide, but greater than that of the essential oil of muña.
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(Universidad Privada Norbert Wiener, 2015) Cerga Rodriguez, Leslie Alexandra; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of essential oils from Cinnamomum zeylanicum (cinnamon) and Syzygium aromaticum (clove) in comparison to 2% Chlorhexidine Gluconate against Enterococcus faecalis strains. The study was experimental, prospective, longitudinal, and analytical. The essential oils from these plants were obtained using the steam distillation method, utilizing the bark of Cinnamomum zeylanicum (cinnamon) and dried buds of Syzygium aromaticum (clove) at concentrations of 1%, 5%, 25%, 50%, 75%, and 100%. For the experiment, Enterococcus faecalis (ATCC 29212) was reactivated and then cultured on Petri dishes (15 cm in diameter) containing Mueller Hinton agar with 6 mm diameter wells, where approximately 100 µl of the essential oils were added, compared to 2% Chlorhexidine Gluconate as a positive control and Tween 20 as a negative control. The plates were incubated at 37°C to check for bacterial viability and media sterility, and the inhibition zones were measured using a Vernier caliper at 24 and 48 hours. In vitro sensitivity testing yielded the following results: the antimicrobial effect of Cinnamomum zeylanicum (cinnamon) essential oil at its minimum effective concentration (1%) was greater than the antimicrobial effect of Syzygium aromaticum (clove) essential oil at its minimum effective concentration (25%). Additionally, a greater antimicrobial effect was observed with the positive control, 2% Chlorhexidine Gluconate.
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(Universidad Privada Norbert Wiener, 2018-03-05) Gutierrez Ortiz, Candy Genesis; Cupé Araujo, Ana Cecilia
Objective: To determine the antimicrobial action of the triple antibiotic paste (TAP) and its modification with clindamycin at concentrations of 2 µg/ml, 1 µg/ml, 0.5 µg/ml, 0.25 µg/ml, 0.125 µg/ml against Enterococcus faecalis ATCC 29212 strain. Materials and methods: The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the drugs comprising TAP (minocycline, metronidazole, and ciprofloxacin) and MTAP (clindamycin, metronidazole, and ciprofloxacin) were determined using the broth dilution method on E. faecalis strains at concentrations of 2 µg/ml, 1 µg/ml, 0.5 µg/ml, 0.25 µg/ml, and 0.125 µg/ml for each group. Results: Significant turbidity changes were observed for TAP at 2 µg/ml and 1 µg/ml, with the latter being the MIC. No bactericidal effects were observed at both concentrations. On the other hand, the MTAP group showed significant turbidity changes at all concentrations, except for the control group, with the MIC being 0.125 µg/ml and the MBC 0.5 µg/ml, respectively. Conclusions: Both combinations exhibited antimicrobial activity; however, MTAP demonstrated a minimum inhibitory concentration of 0.125 µg/ml and bactericidal concentration of 0.5 µg/ml, effectively inhibiting the growth of Enterococcus faecalis at low concentrations.
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(Universidad Privada Norbert Wiener, 2014) Zavala Vega, Luis Antonio; Malpartida Quispe, Federico Martin
The presence of bacteria in the root canal induces chronic periapical disease. Therefore, one of the most important objectives of endodontic treatment is to eliminate all microorganisms from the root canals, using both irrigating solutions and intra-canal medication between sessions, especially in cases of endodontic retreatment, due to the presence of persistent bacteria that may resist various commonly used intra-canal medications. Enterococcus faecalis are the most frequently isolated bacteria from teeth with endodontic failure and the main contributors to persistent apical periodontitis. The objective of this study was to evaluate the inhibitory effect of 2% chlorhexidine gel and calcium hydroxide mixed with various vehicles (2% chlorhexidine solution, camphorated paramonochlorophenol, and saline solution) against Enterococcus faecalis, using the agar well diffusion method. Fifty Petri dishes with brain heart agar were prepared, with each dish containing 4 wells saturated with intra-canal medications. The samples were incubated at 37°C, and measurements of bacterial inhibition zones were recorded at 1, 7, and 15 days. The data were processed using the analysis of variance statistical test, concluding that 2% chlorhexidine gel has greater antibacterial efficacy than calcium hydroxide combined with various vehicles (2% chlorhexidine solution, camphorated paramonochlorophenol, and saline solution) after 1, 7, and 15 days against Enterococcus faecalis.
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Antibacterial action of irrigating compounds using 5% sodium hypochlorite, 17% ethylenediaminetetraacetic acid and 2% chlorhexidine gluconate, against enterococcus faecalis culture strains through an in vitro study Lima-2022
(Universidad Privada Norbert Wiener, 2022-12-07) Salcedo Yanapa, Camila Ximena; Huamani Caquiamarca, Yuliana Esther
The main objective of this study was to evaluate the antibacterial action of the irrigant compounds Sodium Hypochlorite at 5%, Ethylenediaminetetraacetic Acid (EDTA) at 17%, and Chlorhexidine Gluconate at 2% against Enterococcus faecalis strains. This in vitro experimental study, cross-sectional, and prospective, included root canal irrigants and 15 wells per group in Petri plates inoculated with Enterococcus faecalis. To assess the antibacterial action, the agar diffusion method was used, and the samples were incubated at 37°C. After 24 hours, the bacterial inhibition zones were measured and recorded. Kruskal-Wallis test was used for statistical analysis. The results showed that EDTA at 17% had the highest antibacterial action against E. faecalis, presenting an inhibition zone of 25 mm, followed by Chlorhexidine Gluconate at 2% with an inhibition zone of 23 mm, and Sodium Hypochlorite at 5% with an inhibition zone of 11 mm. The study concluded that EDTA at 17% has significantly higher antibacterial action compared to Chlorhexidine Gluconate at 2%, followed by Sodium Hypochlorite at 5% against Enterococcus faecalis.
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Antibacterial activity of propolis and its combination with calcium hydroxide on Enterococcus Faecalis. in vitro study 2021.
(Universidad Privada Norbert Wiener, 2022-06-10) Perez Llantoy, Linda Katherine; Vilchez Bellido, Dina
The purpose of this study was to determine the antibacterial activity of 30% propolis extract and its combination with calcium hydroxide against Enterococcus faecalis ATCC 19433 strains. The sample consisted of Enterococcus faecalis ATCC 19433 cultures, divided into four groups of 25 cultures: three experimental pastes (calcium hydroxide "PrevestDenPro" 70%, 30% propolis extract, and calcium hydroxide 70% combined with 30% propolis) and one negative control (distilled water), with inhibition halos measured at 24 and 48 hours. The results showed antibacterial activity in all three experimental pastes (inhibition halos > 0): 70% calcium hydroxide (M: 7.164 and M: 7.664), 70% calcium hydroxide + 30% propolis (M: 7.804 and M: 8.184), and 30% propolis extract (M: 12.848 and M: 13.428) at 24 and 48 hours, respectively. Significant differences were found between the inhibition halos at 24 and 48 hours for each substance (p value < 0.05). Pairwise comparisons showed significant differences between propolis extract and calcium hydroxide (sig: 0.00), and between calcium hydroxide + propolis extract and propolis extract (sig: 0.00), with no significant difference between calcium hydroxide and calcium hydroxide + propolis extract (sig: 0.080 and 0.484 at 24 and 48 hours). In conclusion, there is antibacterial effectiveness of 30% propolis extract and its combination with calcium hydroxide against Enterococcus faecalis ATCC 19433 strains at 24 and 48 hours, with 30% propolis extract showing the highest antibacterial activity.
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Antibacterial effect of irrigating compounds using 5.25% sodium hypochlorite, 40% citric acid and 2% chlorhexidine gluconate against Enterococcus faecalis strain - in vitro - Lima, 2023
(Universidad Privada Norbert Wiener, 2023-12-04) Pomacaja Ponce, Yuri Evelin; Huamani Caquiamarca, Yuliana Esther
The objective of this study was to determine the antibacterial effect of irrigating compounds against the Enterococcus faecalis strain. The methodology employed was applied, with an analytical experimental design. A sample of 80 plates inoculated with the E. faecalis strain was used, which were treated with each of the irrigating compounds. The diameter of the inhibition zones was evaluated at three different times: 24, 48, and 72 hours, using the Duraffourd scale as the classification criterion. The results showed that sodium hypochlorite-based irrigating compounds at 5.25% had an average inhibition zone diameter of 11.44 ± 2.05 mm, classifying them as "sensitive." Citric acid-based compounds at 40% exhibited an average inhibition zone diameter of 24.41 ± 2.18 mm, classifying them as "highly sensitive." Furthermore, digluconate of chlorhexidine 2% based compounds presented an average inhibition zone diameter of 19.39 ± 1.18 mm, classifying them as "very sensitive." In conclusion, the evaluated irrigating compounds demonstrated an antibacterial effect against the E. faecalis strain.
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Antibacterial effect of Trimix-MP paste and Fortrimax paste on Enterococcus faecalis strain: in vitro study, Lima 2017
(Universidad Privada Norbert Wiener, 2018-12-11) Zevallos Yanqui, Angélica; Ordoñez Lopez, Carmen Jenny
The objective of this research was to determine the antibacterial effect of Fortrimax paste in comparison with Trimix-MP paste on the Enterococcus Faecalis ATCC® 29212 strain. This study was developed in vitro at the microbiology laboratory of Universidad Privada Norbert Wiener, using the modified well diffusion method. The study population consisted of 160 Petri dishes containing Mueller Hinton agar, inoculated with Enterococcus faecalis, and distributed into 40 Petri dishes for each sample. A 6mm well was perforated in each plate, where the antibiotic paste was deposited, and incubated at 37ºC. The measurements of inhibition halos were taken at 24, 48 hours, and 7 days. The data were processed using the Mann-Whitney statistical test for comparisons. The Fortrimax paste showed a greater inhibitory effect, with an average of 36.5mm at 24 hours, 39.06mm at 48 hours, and 39.79mm at 7 days, showing an increasing trend in inhibition. The Trimix paste showed 30.38mm at 24 hours, 29.70mm at 48 hours, and 29.41mm at 7 days of inhibition. Finally, the positive control paste (Calcium Hydroxide) showed 13.15mm at 24 hours, 13.08mm at 48 hours, and 12.90mm at 7 days, demonstrating a lower inhibitory effect. The study concluded that Fortrimax paste had a better antibacterial effect and higher inhibition compared to Trimix paste and Calcium Hydroxide paste on the Enterococcus faecalis strain.
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Bacterial inhibitory effect of essential oil of Eucalyptus Globulus against standardized strain of Enterococcus Faecalis and Phorphyromonas Gingivalis in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2024-08-05) Girón Guayanay, Laura Paola; Velasquez Velasquez, Roxana Pilar
This research aimed to determine the antibacterial inhibitory effect of Eucalyptus globulus essential oil against standardized strains of Enterococcus faecalis and Porphyromonas gingivalis in an in vitro study, Lima - 2023. A quantitative methodology with an analytical focus was used, combining practical application and a prospective quasi-experimental design of a longitudinal nature. A sample size of 160 agar plates was employed, distributed into two main groups: 80 plates for the evaluation of Enterococcus faecalis strains and 80 for the evaluation of Porphyromonas gingivalis. These were further subdivided into four subgroups of 20 Petri plates each. Each subgroup was assigned a specific treatment, which included 20 plates with Eucalyptus globulus essential oil compounds at 50%, 20 with compounds at 75%, 20 with compounds at 100%, and 20 plates with physiological serum as a control. A specific evaluation form was used for the study, where the data generated during the laboratory activities were recorded. The results concluded that there were significant differences in the antibacterial inhibitory effect of Eucalyptus globulus essential oil against standardized strains of Enterococcus faecalis and Porphyromonas gingivalis (p <0.001).
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Efectividad antibacteriana de los cementos obturadores de uso endodóntico: un estudio in vitro
(Universidad Privada Norbert Wiener, 2025-12-16) Chicore Solis, Pedro; Llerena Meza de Pastor, Verónica Janice
Introducción: El éxito del tratamiento endodóntico depende de la calidad del sellado apical y de la capacidad de los cementos obturadores para evitar la reinfección del sistema de conductos radiculares. Enterococcus faecalis es una de las bacterias más frecuentes en los fracasos endodónticos por su resistencia y capacidad de formar biopelículas. El objetivo de este estudio fue comparar la efectividad antibacteriana de los cementos obturadores Grossman®, MTA Fillapex®, Vioseal® y Bio-C Sealer® frente a E. faecalis a las 24, 72 y 168 horas. Métodos: Se desarrolló un estudio experimental in vitro utilizando la técnica de difusión en agar. Se midieron los halos de inhibición producidos por cada cemento y los datos se analizaron mediante estadística descriptiva y la prueba de Kruskal-Wallis con un nivel de significancia de p < 0.05. Resultados: Los resultados mostraron diferencias significativas entre los materiales en los tres tiempos evaluados (p < 0.001). Bio-C Sealer® presentó la mayor actividad antibacteriana en todos los periodos (14.34 ± 2.40 mm a 24 h; 16.74 ± 3.63 mm a 72 h; 15.74 ± 3.81 mm a 168 h), mientras que MTA Fillapex® evidenció una acción retardada. Conclusiones: Se concluye que Bio-C Sealer® presenta una efectividad antibacteriana superior y sostenida frente a E. faecalis, atribuida a su composición biocerámica y liberación continua de iones de calcio, lo que respalda su uso clínico en la obturación endodóntica por su eficacia antimicrobiana y biocompatibilidad.
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Efectividad antimicrobiana de la clorhexidina al 2% e hipoclorito de sodio al 2,5% y 5% frente a una bacteria (Enterococcus faecalis) y un hongo (Candida albicans) Estudio in vitro, Lima 2024
(Universidad Privada Norbert Wiener, 2025-06-30) Huaman Pineda, Eva; Torres Pariona, David Arturo
El presente estudio planteó como objetivo determinar la efectividad antimicrobiana de la clorhexidina al 2% e hipoclorito de sodio al 2.5% y 5% frente a una bacteria (Enterococcus faecalis) y un hongo (Candida Albicans). Es un estudio de método hipotético deductivo, cuantitativo de tipo aplicada y diseño experimental. Se activaron y replicaron las especies microbianas: bacteria Enterococcus faecalis (cepa ATCC 29212) y el hongo Candida albicans (cepa ATCC 90028). Para ello, se emplearon 30 placas de Petri (15 para cada microorganismo) con cultivos estandarizados, las cuales se incubaron a 37°C en condiciones controladas. Una vez sembradas las cepas bacterianes se produjeron 4 pozos de 6 mm de diámetro equidistantes en cada placa Petri, en los cuales se agregó 25 μL de cada sustancia y dejada por un plazo de 72 horas a una temperatura de 37°, siendo únicamente evaluados la efectividad antimicrobiana en el transcurso de las 24, 48 y 72 horas. La mayor efectividad antimicrobiana la presento la clorhexidina solución al 2%, seguido de la clorhexidina gel al 2%, hipoclorito de sodio al 5% e hipoclorito de sodio al 2.5%. Se concluye que la clorhexidina al 2% presento mayor efectividad antimicrobiana frente a una bacteria (Enterococcus faecalis) y un hongo (Candida albicans).
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effect of plant extracts mixed with an endodontic sealant on the growth of oral pathogens in the root canal an in vitro study
(Universidad Privada Norbert Wiener, 2023-11-18) Diaz León, Marlon Omar; Guevara Sotomayor, Juan César
This research aimed to evaluate the effect of plant extracts (thyme, copaiba, and annatto) mixed with an endodontic sealer on the growth of oral pathogens in the root canal, such as Enterococcus faecalis and Streptococcus mutans. An in vitro experimental study with an applied analytical methodology was designed for this purpose. A total sample of 80 Petri dishes was divided into two main groups of 40 each for each microorganism, and further subdivided for each treatment and its control group. The results showed that all three plant extracts mixed with a zinc-based endodontic sealer had an inhibitory effect on the growth of the oral pathogens Enterococcus faecalis and Streptococcus mutans. The inhibition zones around the microorganisms were greater than zero in both the first evaluation at 24 hours and at 48 hours. Additionally, the highest effect was observed in the endodontic sealer added with thyme, showing a greater effect than the pure endodontic sealer for Enterococcus faecalis with 12.75 ± 1.64mm and 12.63 ± 1.65mm for the 24 and 48-hour evaluations, and for Streptococcus mutans, 19.59 ± 2.07mm and 19.47 ± 2.06mm for the 24 and 48-hour evaluations, respectively. Therefore, it was concluded that the evaluated plant extracts mixed with endodontic sealer do present an antibacterial effect.