Examinando por Materia "Porphyromonas gingivalis"

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(Universidad Privada Norbert Wiener, 2017) Quichca Mendoza, Juán Carlos; Aguirre Morales, Anita Kori
Throughout history, periodontal disease has been present, with multiple treatments using natural derivatives to address this condition. Therefore, the objective of this in vitro study was to determine the efficacy of Minthostachys mollis (muña) essential oil and 0.12% Chlorhexidine in inhibiting the growth of Porphyromonas gingivalis. The sample consisted of 40 Petri dishes inoculated with Porphyromonas gingivalis strains, and the agar diffusion method using paper discs was employed. The discs were impregnated with 20 µl of 50% and 100% Minthostachys mollis (muña) essential oil, 0.12% Chlorhexidine, and distilled water. The Petri dishes, inoculated and medicated, were incubated for 24 hours at 37°C and were removed only to measure the inhibition halos at 24 and 48 hours. The data were processed using analysis of variance (ANOVA). Conclusion: The essential oil of Minthostachys mollis (muña) at 50% and 100% were less effective than 0.12% Chlorhexidine in inhibiting the growth of Porphyromonas gingivalis at both 24 and 48 hours.
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(Universidad Privada Norbert Wiener, 2015) Carhuaricra Soto, Yudiht Abigail; Malpartida Quispe, Federico Martin
In periodontal disease, various microorganisms are involved, one of which is Porphyromonas gingivalis, an obligate anaerobic, Gram-negative bacterium that is predominantly found in chronic periodontitis, and is commonly eliminated by 0.12% chlorhexidine. The purpose of this study was to determine the inhibitory effect of Rosmarinus officinalis (rosemary) essential oil and 0.12% chlorhexidine on Porphyromonas gingivalis, using the agar diffusion method with discs, as an effort to find an alternative, natural, effective, and economical antimicrobial substance against this bacterium. For this, Porphyromonas gingivalis strains were inoculated in 40 Petri dishes containing Mueller Hinton agar. Then, three discs were placed on each plate, impregnated with 20 µl of 0.12% chlorhexidine, Rosmarinus officinalis essential oil, and distilled water as a negative control. The plates were incubated at 37°C in an anaerobic jar for the duration of the study, being removed only to measure the inhibition halos generated at 24 and 48 hours. The data were processed using SPSS software, and the Student's T-test was applied. It was concluded that Rosmarinus officinalis (rosemary) essential oil exhibited an inhibitory effect against Porphyromonas gingivalis, and 0.12% chlorhexidine had a greater inhibitory effect than the essential oil against this bacterial strain.
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Acción antibacteriana del extracto etanólico de Azadirachta Indica (Neem) en comparación con el Gluconato de Clorhexidina al 0.12% con Cloruro de Cetilpiridinio 0,05% frente a las cepas de Porphyromonas gingivalis. Estudio in vitro. Lima. 2023
(Universidad Privada Norbert Wiener, 2024-10-04) Rosas Benites, Viviangelli Vanessa; Morante Maturana, Sara Angélica
Con el propósito de aportar un enfoque terapéutico diferente y natural, se efectuó una investigación en la que se evaluó dicha diferencia en la acción antibacteriana in vitro entre el extracto etanólico del Azadirachta indica comparado con la clorhexidina al 0,12% con CPC al 0,05% sobre las cepas de Porphyromonas gingivalis. Cabe resaltar que la muestra estuvo conformada por 15 de muestra por sustancia de prueba; fraccionadas en grupos: tres grupos con el extracto etanólico al 25%, 75% y 100% y dos grupos con los respectivos controles tanto positivo como negativo que, en total se emplearon 75 placas de agar Schaedler enriquecido Es una investigación hipotética/ deductivo, desde un enfoque cuantitativo, de tipo aplicada, diseño experimental in vitro. Los resultados demostraron que existe acción antibacteriana significativo en relación con la medida de Duraffourd. Tratándose en el caso de la clorhexidina al 0,12% y CPC al 0,05% los valores medios después de transcurridas 24 h (14,34 mm), 48 h (14,22 mm) y 72 h (14,11mm); en cuanto al extracto etanólico al 100% presentó promedios, a las 24 h (11,38 mm), 48 h (11,27 mm) y 72 horas (11,15 mm); el extracto etanólico al 75% registró valores estadísticos, en 24 h (9,19 mm), 48 h (9,08 mm) y 72 h (8.97 mm); a la vez el extracto etanólico al 25% no presentó acción antibacteriana. Se llegó a la conclusión, que el gluconato de clorhexidina al 0,12% y CPC al 0,05% presenta mayor actividad antibacteriana en contraste con los extractos etanólicos en estudio.
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Actividad antibacteriana del extracto etanólico de equisetum arvense (cola de caballo) sobre porphyromona gingivalis: estudio in vitro en Lima, 2024
(Universidad Privada Norbert Wiener, 2025-02-03) Rojas Ventura, Milagros Kelly; Marroquín García, Lorenzo Enrique
La investigación estableció como objetivo “Determinar la actividad antibacteriana del extracto etanólico de Equisetum arvense sobre la Porphyromona gingivalis, estudio in vitro en Lima, 2024”. Se empleó un diseño experimental in vitro del cultivo de cepas estándares de Porphyromona gingivalis. La muestra incluyó a 10 placas Petri cada una con 5 discos en concentraciones al 6,25%, 12,5%, 25%, 50% y 100% del extracto etanólico de Equisetum arvense. La recolección de datos se llevó a cabo mediante la técnica de observación. Los resultados obtenidos evidenciaron una diferencia significativa en la actividad antibacteriana del extracto etanólico de Equisetum arvense frente a Porphyromona gingivalis in vitro, según el análisis de varianza (ANOVA), en relación con la concentración empleada. En consecuencia, se rechaza la hipótesis nula, concluyendo que existen diferencias significativas en la actividad antibacteriana en relación con la concentración utilizada (p ≤ 0.05).
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Antibacterial activity of eucalyptus globulus essential oil compared to 0.12% chlorhexidine gluconate in inhibiting porphyromona gingivalis in vitro.
(Universidad Privada Norbert Wiener, 2021-05-05) Feldmuth Gonzales, Henry Jefferson; Girano Castaños, Jorge Alberto
Objective: The objective of this research was to determine the antibacterial activity of eucalyptus essential oil (Eucalyptus globulus) compared to 0.12% chlorhexidine digluconate in inhibiting Porphyromonas gingivalis in vitro. Methodology: A quasi-experimental design was developed, considering the antibacterial agent variable in 4 concentrations of eucalyptus essential oil (10%, 25%, 50%, 100%) and 0.12% chlorhexidine digluconate. Porphyromonas gingivalis bacteria were cultured in media using a sample of 40 plates. Results: This research found that Eucalyptus globulus exhibited greater antibacterial activity at all concentrations than 0.12% chlorhexidine, both at 24 and 48 hours against Porphyromonas gingivalis. The smallest inhibition halo was found for 10% eucalyptus with 18.65 mm, and the largest was for 100% eucalyptus at 48 hours with 29.55 mm. Conclusion: Eucalyptus essential oil (Eucalyptus globulus) has significantly greater antibacterial activity than 0.12% chlorhexidine digluconate in inhibiting Porphyromonas gingivalis in vitro.
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Bacterial inhibitory effect of essential oil of Eucalyptus Globulus against standardized strain of Enterococcus Faecalis and Phorphyromonas Gingivalis in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2024-08-05) Girón Guayanay, Laura Paola; Velasquez Velasquez, Roxana Pilar
This research aimed to determine the antibacterial inhibitory effect of Eucalyptus globulus essential oil against standardized strains of Enterococcus faecalis and Porphyromonas gingivalis in an in vitro study, Lima - 2023. A quantitative methodology with an analytical focus was used, combining practical application and a prospective quasi-experimental design of a longitudinal nature. A sample size of 160 agar plates was employed, distributed into two main groups: 80 plates for the evaluation of Enterococcus faecalis strains and 80 for the evaluation of Porphyromonas gingivalis. These were further subdivided into four subgroups of 20 Petri plates each. Each subgroup was assigned a specific treatment, which included 20 plates with Eucalyptus globulus essential oil compounds at 50%, 20 with compounds at 75%, 20 with compounds at 100%, and 20 plates with physiological serum as a control. A specific evaluation form was used for the study, where the data generated during the laboratory activities were recorded. The results concluded that there were significant differences in the antibacterial inhibitory effect of Eucalyptus globulus essential oil against standardized strains of Enterococcus faecalis and Porphyromonas gingivalis (p <0.001).
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Comparison of antibacterial activity of essential oils from Schinus molle L. (molle) and Thymus vulgaris (thyme) with chlorhexidine gluconate 0.12% against Porphyromonas gingivalis: in vitro study
(Universidad Privada Norbert Wiener, 2019-01-17) Neira Jara, Vanessa Evelin; Salcedo Rioja, Mercedes Rita
This research study was conducted in vitro with the objective of comparing the antibacterial activity of essential oils from Schinus molle L. (molle) and Thymus vulgaris (thyme) with 0.12% chlorhexidine gluconate against Porphyromonas gingivalis. This experimental study was carried out at the National University of San Marcos in the Microbiology Laboratory of the Faculty of Dentistry. The sample consisted of 40 Petri dishes where the essential oils Thymus vulgaris at 100% and 50%, Schinus molle L. at 100% and 50% were used, compared with 0.12% chlorhexidine gluconate, and dimethyl sulfoxide (DMSO) as a negative control. The disk diffusion method was used, and the dishes were incubated for 15 days at 37°C. The inhibition halos were measured only once at the time of measurement. Data analysis was processed using the Scheffé's Post Hoc statistical test. The results showed that, of the two oils, thyme had the greatest effect on Porphyromonas gingivalis, while molle had the least effect. Both thyme and molle essential oils exhibited greater antibacterial activity than 0.12% chlorhexidine gluconate.
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Comparison of the antibacterial effectiveness of irrigants based on 0.12% chlorhexidine and ozonized sunflower oil on porphyromonas gingivalis strains - in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2023-06-08) Blas Huaroc, Esteban Abraham; Huayllas Paredes, Betzabé
The objective was to determine the antibacterial effectiveness of irrigants based on 0.12% Chlorhexidine and ozonized sunflower oil against Porphyromonas gingivalis strains. This was an applied research study with an analytical method, quantitative focus, and an in-vitro experimental design, where microorganisms were cultured on 40 Petri dishes containing agar. The results showed significant antibacterial effects of both 0.12% Chlorhexidine and ozonized sunflower oil, according to the Duraffourd Scale. Chlorhexidine 0.12% showed average inhibition zones of 17.58 mm at 24 hours, 17.56 mm at 48 hours, and 17.55 mm at 72 hours. On the other hand, ozonized sunflower oil showed inhibition zones of 36.5 mm at 24 hours, 36.49 mm at 48 hours, and 36.47 mm at 72 hours. It was concluded that ozonized sunflower oil had a better antibacterial effect than 0.12% Chlorhexidine against Porphyromonas gingivalis strains.
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Comparison of the antibacterial effectiveness of three commercial brands of mouthwashes (Colgate plax soft mint, Oral-b Complete and Perio.AID-Intensive Care) on strains of Porphyromonas gingivalis ATCC 33277 for the adjuvant treatment of periodontal disease. in vitro study year 2021.
(Universidad Privada Norbert Wiener, 2022-05-04) Herrera Martínez, Elidex Stefany; Huayllas Paredes, Betzabé
Objective: To evaluate the in vitro antibacterial effectiveness of three commercial mouthwash brands against the bacterium Porphyromonas gingivalis as an adjunctive treatment for periodontal disease. This study aims to compare the inhibitory growth diameters of this bacterium from the mouthwash solutions Colgate Plax Soft Mint, Oral-B Complete, and Perio.Aid-Intensive Care over time. Methodology: The research method was hypothetical-deductive, applied research with a quantitative approach, and an experimental in vitro, analytical, longitudinal, and prospective design. A total of 88 BHI agar plates with Porphyromonas gingivalis ATCC 33277 were established as the study sample. Results: At the end of the study, all three mouthwash brands showed antibacterial effectiveness at 24, 48, and 72 hours against Porphyromonas gingivalis strains. The Oral-B Complete mouthwash presented the smallest inhibition zone, with an average of 10.99 mm at 24 hours, while the Perio.Aid-Intensive Care showed the greatest inhibition with 30.49 mm at 72 hours. Conclusion: A significant difference was found in the antibacterial effectiveness of the three mouthwash brands in inhibiting the periodontal pathogen P. gingivalis ATCC 33277 for the adjunctive treatment of periodontal disease in vitro, with Perio.Aid-Intensive Care showing the highest inhibition at 24, 48, and 72 hours.
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Efectividad del aceite esencial de mintostachys mollis griseb en concentraciones de 25%, 50% y 100% frente a porphiromonas gingivales ATCC ® 33277™ en el laboratorio scientific quality, 2023
(Universidad Privada Norbert Wiener, 2025-05-19) Altamirano Gutierrez., Rosario Janin; Guevara Sotomayor, Juan César
La actual investigación propuso determinar la efectividad del aceite esencial de Mintostachys mollis Griseb en concentraciones de 25%, 50% y 100% frente a Porphiromonas gingivales ATCC® 33277™. Se realizó una investigación cuantitativa, aplicado, de tipo experimental, transversal con alcance descriptivo; se analizaron 08 muestras para ver el impacto de las niveles del aceite esencial de Mintostachys mollis frente a Porphyromonas gingivalis ATCC 33277 a las 24, 48 y 72 horas de examen. Se pudo evidenciar que los aceites esenciales de Mintostachys mollis al 100% y 50% presentaron una media halo de bloqueo de 14,50 ± 1,068 mm y de 12,54 ± 0,465 mm, al 25% no registró actividad antibacteriana en este periodo de tiempo, control positivo, ciprofloxacino de 500mg, una media halo de bloqueo de 32,04 ± 0,830 mm a las 24 horas respectivamente, a las 48 horas las concentraciones al 100% y 50% presentaron una media halo de bloqueo de 14,11 ± 1,050 mm y de 12,23 ± 0,497 mm, al 25% no registró actividad antibacteriana, el ciprofloxacino de 500mg, obtuvo una media halo de bloqueo de 31,74 ± 0,794mm, a las 72 horas las concentraciones al 100% y 50% presentaron una media halo de bloqueo de 13,82 ± 1,065 mm y de 11,93 ± 0,494 mm al 25% no registró actividad antibacteriana, el ciprofloxacino de 500mg, obtuvo una media halo de bloqueo 31,42 ± 0,774mm. Frente a Porphyromonas gingivalis ATCC 33277, se puede concluir que los promedios de los aceites esenciales de Minthostachys mollis al 25% no ningún efecto antibacteriano, al 50% y 100% tienen efecto bacteriano pero menor que la actividad biocida del Ciprofloxacino de 500mg, a las 24, 48 y 72 horas de estudio.
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Efecto antimicrobiano del ajo (Allium sativum) sobre las cepas de Porphyromonas gingivalis, 2024
(Universidad Privada Norbert Wiener, 2025-05-13) Zurita Campos, Marleny Judtih; Gómez Carrión, Christian Esteban
El presente estudio buscó determinar el efecto antibacteriano del extracto del ajo (Allium sativum) sobre cepas de P. gingivalis, 2024. Se elaboró una investigación cuantitativa, aplicada, experimental, longitudinal y prospectivo; se analizaron 10 placas Petri por grupo de tratamiento, en un total de 40 placas; utilizando un vernier. Diseñado específicamente para medir la unidad de longitud, este instrumento calibrado es confiable debido a su certificación ISO 9001 y la marca MITUTOYO, modelo 500-157-30. Las zonas de inhibición se documentaron en un formulario de recopilación de información diseñado específicamente a la investigación; se pudo evidenciar que los extractos de Allium sativum al 100%, 75% y 50% frente a Porphyromonas gingivalis ATCC 33277 presentaron un promedio de halo de inhibición de 14,59 ± 0,9836 mm; 11,89 ± 0,9310 mm y 10,88 ± 1,5450 mm, respectivamente, a las 24 horas de estudio; 14,43 ± 0,9793 mm; 11,70 ± 0,9608 mm y 10,72 ± 1,5481 mm, a las 48 horas y 14,25 ± 0,9764 mm; 11,50 ± 0,9540 mm y 10,55 ± 1,5413 mm, respectivamente, a las 72 horas de estudio; por otro lado, el digluconato de clorhexidina al 0,12 % presentó un halo de inhibición de 13,78 ± 1,1936 mm; 13,61± 1,2119 mm y 13,43 ± 1,2280 mm a las 24,48 y 72 horas; para los extractos de Allium sativum al 100%, 75% y 50% y el digluconato de clorhexidina al 0,12% (p<0,05); se concluye que existe efecto antibacteriano del extracto del ajo (Allium sativum) sobre cepas de Porphyromonas gingivalis, 2024.
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Evaluación in vitro de la actividad antimicrobiana del aceite esencial de eucalyptus sp. (eucalipto) y su comparación con el gluconato de clorhexidina al 0,12% frente a porphyromonas gingivalis
(Universidad Privada Norbert Wiener, 2025-04-30) Quispe Rios, Sergio Joel; Morante Maturana, Sara Angelica
El presente trabajo de investigación tuvo como objetivo: Determinar el efecto antibacteriano del aceite esencial de Eucalipto según el halo de inhibición en concentraciones de 100, 50, 25 y 12.5% comparado a la “clorhexidina al 0,12% frente a la Porphyromonas gingivalis”; con una metodología hipotética- deductiva, utilizando un enfoque cuantitativo, de tipo básica y de diseño experimental, la población estará compuesta por tres grupos consistente en dieciocho placas Petri con cepas de Porphyromonas gingivalis recopilados en una muestra de 10 placas Petri en un muestreo será de tipo probabilístico intencional, los resultados de la prueba de Friedman en todos los resultados se presenta un p. valor de 0.000 (< al 0.05%). Concluyendo que si existe diferencia significativa del efecto antibacteriano del aceite esencial de Eucalipto según el halo de inhibición en concentraciones de 100, 50, 25 y 12.5% comparado a la clorhexidina al 0,12% frente a la Porphyromonas gingivalis.