Examinando por Materia "Streptococcus mutans"

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(Universidad Privada Norbert Wiener, 2015) Carrión Reyes, Gueraldin Mercedes; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the ethanolic extract of Bidens pilosa (Amor seco) compared to Colgate Plax® and 0.12% Chlorhexidine Gluconate against strains of Streptococcus mutans. The study was experimental, prospective, longitudinal, and analytical. The population consisted of Streptococcus mutans strains (ATCC 25175), and the sample consisted of 40 Petri dishes. The strains were reactivated and cultured on Blood Agar, where 6 mm diameter wells were made, and approximately 100 µl of the ethanolic extract of Bidens pilosa (Amor seco) were added in the following concentrations: 0.8/10, 1/10, and pure. The results were compared with 0.12% Chlorhexidine Gluconate as a positive control, distilled water as a negative control, and Colgate Plax® mouthwash. The results showed that the 0.8/10 and 1/10 concentrations of the ethanolic extract of Bidens pilosa (Amor seco) did not present any inhibition zone at any time. However, the pure ethanolic extract produced an average inhibition zone of 11.98 mm at 24 hours and 11.80 mm at 48 hours. Furthermore, a stronger inhibitory effect was observed with the positive control, 0.12% Chlorhexidine Gluconate, and a lesser inhibitory effect compared to Colgate Plax® mouthwash against the Streptococcus mutans (ATCC 25175) strain.
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(Universidad Privada Norbert Wiener, 2015) Juscamaita Gutierrez, Elizabeth Stefany; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the essential oil of Eucalyptus globulus compared to 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash on the bacterial inhibition of Streptococcus mutans. The study was experimental, cross-sectional, prospective, and analytical, with an explanatory level, conducted in a laboratory. The population consisted of Streptococcus mutans (ATCC 25175) strains, and the sample included 40 Petri dishes. The culture medium used was Blood Agar, and 6 mm diameter wells were made in each Petri dish using a sterile punch. The essential oil of Eucalyptus globulus at concentrations of 25%, 50%, and 100%, 0.12% Chlorhexidine Gluconate, Colgate Plax® mouthwash, and Tween 20 were added to the wells. The results showed that the 25%, 50%, and 100% concentrations of Eucalyptus globulus essential oil produced inhibition halos with average diameters of 6.88 mm, 9.61 mm, and 12.65 mm at 24 hours, and 6.88 mm, 9.53 mm, and 11.90 mm at 48 hours, respectively, which were smaller than the inhibition halos produced by 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash at both 24 and 48 hours against the Streptococcus mutans (ATCC 25175) strain.
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(Universidad Privada Norbert Wiener, 2017-11-20) Cueva Rosales, Javier; Pareja Cuadros, Elizabeth Irene
The purpose of this research was to assess the antimicrobial activity of rosemary essential oil (Rosmarinus officinalis) against the growth of Streptococcus mutans ATCC 25175 "in vitro". For the microbiological analysis, 100% rosemary essential oil was used and the agar well diffusion method was applied. Thirty Petri dishes with Muller Hinton agar were prepared, each containing a 6 mm diameter well saturated with rosemary essential oil and 0.12% chlorhexidine (DENTODEX®). The samples were incubated at 37°C and were only removed to measure and record the bacterial inhibition halos after 72 and 168 hours. The essential oil was compared with 0.12% chlorhexidine gluconate as a positive control for Streptococcus mutans ATCC 25175, and sterile distilled water was used as the negative control. The data were processed using SPSS, and the T-test was applied. The study concluded that Rosmarinus officinalis (rosemary) essential oil exhibited "in vitro" antibacterial activity against Streptococcus mutans ATCC 25175 at 72 hours, and 0.12% chlorhexidine showed greater antibacterial activity than the essential oil against this bacterial strain at 168 hours.
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(Universidad Privada Norbert Wiener, 2018-04-04) Luis Barrientos, Angel Jorge; Pareja Cuadros, Elizabeth Irene
The objective of this research was to assess the antibacterial activity of the essential oil of Cinnamomum zeylanicum (cinnamon) compared to 0.12% chlorhexidine against Streptococcus mutans ATCC 25175 "in vitro". The study was experimental, prospective, and longitudinal. The essential oil was obtained using the steam distillation method from the bark of Cinnamomum zeylanicum (cinnamon). For microbiological analysis, the essential oil was used at 100%, and the well-agar diffusion method was applied. Thirty Petri dishes with Muller Hinton agar were prepared, each with a 6 mm diameter well saturated with cinnamon essential oil and 0.12% chlorhexidine (DENTODEX®). The samples were incubated at 37°C, and inhibition zones were measured and recorded at 72 and 120 hours. The essential oil was compared to 0.12% chlorhexidine as a positive control for Streptococcus mutans ATCC 25175, with sterile distilled water as the negative control. The data were processed using SPSS, and the T-Student statistical test was applied. The study concluded that the essential oil of Cinnamomum zeylanicum (cinnamon) showed stability and antibacterial activity "in vitro" against Streptococcus mutans ATCC 25175 at 72 and 120 hours. Chlorhexidine 0.12% showed greater antibacterial activity than the essential oil at 120 hours.
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(Universidad Privada Norbert Wiener, 2015) Carrión Reyes, Fiorella Isamar; Malpartida Quispe, Federico Martin
The objective of this study was to determine the inhibitory effect of the essential oil of Schinus molle (Molle) compared to 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash in inhibiting bacterial growth of Streptococcus mutans in vitro. The study was experimental, cross-sectional, prospective, and analytical at an explanatory level, conducted in a microbiology laboratory. The population consisted of Streptococcus mutans strains (ATCC 25175), with a sample of 40 Petri dishes. The agar diffusion method was used with 6 mm diameter wells, where 100 µl of Schinus molle (Molle) essential oil at 25%, 50%, and 100%, 0.12% Chlorhexidine Gluconate, Colgate Plax® mouthwash, and Tween 20 were poured. The results showed that the concentrations of 50% and 100% Schinus molle essential oil produced an average inhibition zone of 10.97 mm and 10.46 mm at 24 hours, and 11.06 mm and 10.59 mm at 48 hours, which were smaller than the inhibition zones formed by 0.12% Chlorhexidine Gluconate and Colgate Plax® mouthwash at both times against the Streptococcus mutans (ATCC 25175) strain.
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(Universidad Privada Norbert Wiener, 2017-09-29) Chávez Hidalgo, Diego Andrés; Cupé Araujo, Ana Cecilia
Materials and Methods: Ten toothpaste brands available in the local market were used, with pediatric toothpastes recommended by specialists in pediatric dentistry. Streptococcus mutans (ATCC 25175) was cultured on a specific medium (tryptic soy agar, yeast extract, sucrose, and bacitracin), which is ideal for the recovery and evaluation of Streptococcus mutans, as referenced in studies certifying its suitability. Bacitracin was used to allow only the growth of this bacterium, preventing contamination in our cultures. The agar diffusion method was used, where the agar is inoculated with the bacterial strain to ensure uniform growth. A modification was made to the preparation of the agar, adding 50 ml of sterile lamb blood to create contrast between the agar and inhibition halos. The cylinder-plate technique from the United States Pharmacopeia was employed, which involves using calibrated cylinders (made of surgical steel or porcelain) measuring 10 mm in height and having an external diameter of 8 mm and an internal diameter of 6 mm. These cylinders were placed on the plates with a spacing of 6 cylinders per plate, following the plate's perimeter and ensuring distance between them for the formation of inhibition halos. According to the technique's protocol, 6 Petri dishes were used for each toothpaste evaluated, 6 plates for evaluating the Gold Standard (penicillin plus gentamicin), and another 6 Petri dishes for the negative evaluation. Conclusions: The toothpaste brands sold in the local market showed inhibitory activity against Streptococcus mutans (ATCC 25175), except for one.
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(Universidad Privada Norbert Wiener, 2015) Atencio Chauca, Evelyn Karin; Cupé Araujo, Ana Cecilia
The objective of this study was to determine the inhibitory effect of Vaccinium corymbosum (Blueberry) at 100%, 75%, 50%, 25%, and 0.12% Chlorhexidine Gluconate against the presence of Streptococcus mutans in an in vitro study in Lima, 2015, at 24 and 48 hours. To this end, Streptococcus mutans strains were isolated from saliva collected from a patient and cultured on Petri dishes containing Blood Agar medium, with 6 mm diameter wells where approximately 100 µl of the 100%, 75%, 50%, 25% extracts, 0.12% Chlorhexidine Gluconate, and distilled water were poured. The plates were incubated at 37°C, including two control plates to check the viability of the bacteria and the sterility of the medium. After incubation, the inhibition halos were measured with a Vernier caliper or ruler at 24 and 48 hours. For data analysis, ANOVA and Dunnett tests were used, showing that 0.12% Chlorhexidine Gluconate had a significantly higher inhibitory effect than the 100% Vaccinium Corymbosum extract (P<0.05), and the 75% and 50% Vaccinium Corymbosum extracts (P<0.05). It was concluded that the inhibitory effect of 100% Vaccinium Corymbosum is lower than that of 75% Vaccinium Corymbosum and very similar to 0.12% Chlorhexidine Gluconate in the bacterial culture of Streptococcus mutans at 24 and 72 hours.
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ACTIVIDAD ANTIMICROBIANA DEL EXTRACTO ETANÓLICO DE LAS HOJAS DE Schinus molle L. “Molle
(Universidad Privada Norbert Wiener, 2017) Clemente Sotteccani, Claudia Elizabeth; Chávez Flores, Juana Elvira
El Perú es uno de los países con mayor biodiversidad en el mundo, muchas de sus especies vegetales pueden ser aprovechadas de forma sostenible por la industria, una clara alternativa es el Schinus molle L. “Molle”, un árbol nativo con aplicaciones medicinales, cuyo extracto etanólico presenta propiedades antibacterianas. La presente investigación tuvo como objetivo comprobar la actividad antimicrobiana del extracto etanólico de las hojas de Schinus molle L. “Molle”. Para ello se procedió a obtener el extracto etanólico a concentraciones de 500 y 1000 mg/mL, el gluconato de clorhexidina 0,12 % como control positivo y agua destilada como control negativo. Se realizó el estudio de la actividad antimicrobiana con el método de difusión en disco utilizando la técnica de siembra en superficie, con las soluciones experimentales en condiciones de anaerobiosis por 48 y 72 horas a 37°C, para luego proceder a la lectura de diámetros de halo de inhibición con un vernier. Mediante el análisis cualitativo se determinó la presencia de metabolitos: flavonoides, alcaloides, carbohidrato, esteroides y/o triterpenos, azúcar reductores y compuestos fenólicos. Los resultados se analizaron mediante el porcentaje de inhibición del crecimiento. El extracto etanólico a concentraciones de 500 y 1000 mg/mL, demostró tener actividad antimicrobiana sobre Streptococcus mutans “ATCC 25175” y de la comparación realizada, el gluconato de clorhexidina produjo mayor inhibición. Se concluye que el extracto etanólico de Schinus molle L. “Molle” presenta actividad antimicrobiana sobre Streptococcus mutans “ATCC 25175” y el gluconato de clorhexidina es cualitativamente similar al extracto etanólico de Schinus molle L. “Molle“.
Acceso abierto
ACTIVIDAD ANTIMICROBIANA DEL EXTRACTO ETANÓLICO DE LAS HOJAS DE Schinus molle L. “Molle
(Universidad Privada Norbert Wiener, 2017) Paucar Lopez, Rosmery; Chávez Flores, Juana Elvira
El Perú es uno de los países con mayor biodiversidad en el mundo, muchas de sus especies vegetales pueden ser aprovechadas de forma sostenible por la industria, una clara alternativa es el Schinus molle L. “Molle”, un árbol nativo con aplicaciones medicinales, cuyo extracto etanólico presenta propiedades antibacterianas. La presente investigación tuvo como objetivo comprobar la actividad antimicrobiana del extracto etanólico de las hojas de Schinus molle L. “Molle”. Para ello se procedió a obtener el extracto etanólico a concentraciones de 500 y 1000 mg/mL, el gluconato de clorhexidina 0,12 % como control positivo y agua destilada como control negativo. Se realizó el estudio de la actividad antimicrobiana con el método de difusión en disco utilizando la técnica de siembra en superficie, con las soluciones experimentales en condiciones de anaerobiosis por 48 y 72 horas a 37°C, para luego proceder a la lectura de diámetros de halo de inhibición con un vernier. Mediante el análisis cualitativo se determinó la presencia de metabolitos: flavonoides, alcaloides, carbohidrato, esteroides y/o triterpenos, azúcar reductores y compuestos fenólicos. Los resultados se analizaron mediante el porcentaje de inhibición del crecimiento. El extracto etanólico a concentraciones de 500 y 1000 mg/mL, demostró tener actividad antimicrobiana sobre Streptococcus mutans “ATCC 25175” y de la comparación realizada, el gluconato de clorhexidina produjo mayor inhibición. Se concluye que el extracto etanólico de Schinus molle L. “Molle” presenta actividad antimicrobiana sobre Streptococcus mutans “ATCC 25175” y el gluconato de clorhexidina es cualitativamente similar al extracto etanólico de Schinus molle L. “Molle“.
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Actividad inhibitoria de la Stevia rebaudiana y el xilitol sobre las cepas de Streptococcus mutans in vitro, Lima – 2025
(Universidad Privada Norbert Wiener, 2025-10-23) Escurra Torres, Kelly Samantha; Huayllas Paredes, Betzabe
El presente estudio tuvo como objetivo evaluar la actividad inhibitoria de la Stevia rebaudiana y el xilitol sobre las cepas de Streptococcus mutans in vitro, Lima- 2025. Se empleó un diseño experimental in vitro de enfoque cuantitativo, utilizando 80 discos de cartón absorbente distribuidos en grupos: Stevia (25%, 50%, 100%), xilitol (25%, 50%, 100%), clorhexidina al 0.12% (control positivo) y agua destilada (control negativo). Los discos, impregnados con las soluciones, se incubaron con cepas de S. mutans ATCC 25175, midiendo los halos de inhibición según la escala de Duraffourd (actividad significativa: halo ≥8 mm). Los resultados demostraron que ni Stevia ni xilitol alcanzaron el umbral inhibitorio. Los halos promedio fueron: Stevia 25% (5.49 mm), 50% (6.20 mm) y 100% (sin efecto adicional), xilitol 25% (5.49 mm) y 50% (6.01 mm). En contraste, la clorhexidina mostró un halo de 22.51 mm (p <0.05). Además, las comparaciones entre concentraciones de edulcorantes no revelaron diferencias significativas (p >0.05), confirmando su ineficacia incluso al 50%. Se concluye que, bajo condiciones in vitro, Stevia rebaudiana y xilitol no inhiben el crecimiento de S. mutans, independientemente de su concentración (25%-100%). La clorhexidina mantuvo su superioridad, evidenciando que estos edulcorantes no son alternativas viables como agentes antibacterianos para prevenir caries.
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Antibacterial activity of croton lechleri and stretococcus mutans ATCC 25175 latex-based mouthwash
(Universidad Privada Norbert Wiener, 2022-10-05) Astudillo Aquino, Robert Benjamin; Marroquín García, Lorenzo Enrique
The aim of this project was to evaluate the inhibitory capacity of a mouthwash based on latex from Croton lechleri (dragon’s blood) on the proliferation of Streptococcus mutans ATCC 25175 "in vitro." For the microbiological study, 100% dragon’s blood latex was used, and the agar diffusion method was employed. Sixteen Petri dishes with BHI agar were prepared, each containing a 6 mm diameter well saturated with 0.12% chlorhexidine and dragon’s blood latex. The samples were incubated at 37°C, and inhibition zones were recorded after 24 and 72 hours. Chlorhexidine at 0.12% was used as the positive control for Streptococcus mutans ATCC 25175, and distilled water served as the negative control. Data were analyzed using SPSS, and the T-Student statistical test was applied. The conclusion was that the mouthwash based on Croton lechleri latex (dragon’s blood) exhibits antimicrobial activity "in vitro" against Streptococcus mutans ATCC 25175 at 24 and 72 hours, with chlorhexidine at 0.12% showing superior antibacterial activity.
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Antibacterial effect of ethanolic extract of propolis at 30% and cetylpyridinium chloride at 0.05% plus chlorhexidine digluconate at 0.05% on strains of Streptococcus mutans (ATCC 25175). In Vitro comparative study. 2021.
(Universidad Privada Norbert Wiener, 2022-02-10) Ochoa Concha, Rubila Celeste; Schwan Silva, Ignacio Segundo
This is an in vitro experimental study using two samples: Sample 1, 30% ethanol propolis extract, and Sample 2, cetylpyridinium chloride at 0.05% plus chlorhexidine digluconate at 0.05%, with chlorhexidine digluconate at 0.12% as the control group. Inhibition halos were measured around each of the discs soaked with the antibacterial substances placed on the plates. The plates were incubated for 48 hours, and the halos were measured using a Vernier caliper. The Duraffourd et al. scale (1986) was used for interpreting the antibacterial effect against Streptococcus mutans. The streaking and exhaustion technique was used on the agar. A total of 30 experimental units were plated on blood agar, on which 3 sterile antibiogram discs of 6mm each were placed, inoculated with Streptococcus mutans.
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Antibacterial effect of Stevia rebaudiana, Camellia sinensis and Origanum vulgare against Streptococcus mutans in vitro study, Lima - 2023
(Universidad Privada Norbert Wiener, 2024-06-04) Mamani Turpo, Luz Delia; Chilón Minaya, Lesly Johanna
The aim of this study was to determine the antibacterial effect of three plant-based compounds: Stevia rebaudiana, Camellia sinensis, and Origanum vulgare, against Streptococcus mutans. An in vitro experimental design methodology was used, with an analytical method and applied type. Treatments were applied to Petri dishes with Streptococcus mutans strains, using 10 plates for each treatment and two control groups. The results revealed that, of the three plant-based compounds tested, two showed antibacterial effects against Streptococcus mutans. For Origanum vulgare, inhibition halos of 32.09 mm were obtained at 24 hours and 31.80 mm at 48 hours. For Camellia sinensis, inhibition halos were 25.06 mm at 24 hours and 24.74 mm at 48 hours. However, Stevia rebaudiana did not show an antibacterial effect as its inhibition halos were 0 mm. Therefore, it was concluded that the antibacterial compounds that did show antibacterial effects did not show significant differences compared to the positive control used, 0.12% Chlorhexidine Gluconate (p>0.05).
Acceso abierto
Antibacterial effect of the hydroalcoholic extract of erythroxylum coca lam. (coca) compared to chlorhexidine gluconate 0.12% versus strains of streptococcus mutans. In vitro study. Lima 2021
(Universidad Privada Norbert Wiener, 2021-12-04) Macedo Heredia, Raúl Enrique; Granados Carrera, Julio Cesar
Objetivo: Esta investigación tuvo como objetivo evaluar el efecto antibacteriano del extracto hidroalcohólico de Erythroxylum coca Lam. (coca) comparado con gluconato de clorhexidina al 0,12% contra cepas de Streptococcus mutans in vitro. Metodología: Se realizó un estudio cuantitativo, aplicado, cuasi experimental. La variable principal estudiada fue el agente antibacteriano, comparándose concentraciones de 50%, 75% y 100% del extracto hidroalcohólico de Erythroxylum coca Lam. (coca) con gluconato de clorhexidina al 0,12%. Se desarrollaron cultivos bacterianos de Streptococcus mutans utilizando una muestra de 70 placas. Resultados: El extracto hidroalcohólico de Erythroxylum coca Lam. (coca) mostró efectos antibacterianos contra Streptococcus mutans en las tres concentraciones a las 24 y 48 horas. El mayor halo de inhibición media se observó con una concentración del 50% a las 48 horas (31,01 mm), mientras que el menor se registró con clorhexidina al 0,12% a las 24 horas (10 mm). Conclusión: Existe una diferencia significativa en el efecto antibacteriano del extracto hidroalcohólico de Erythroxylum coca Lam. (coca) en comparación con el gluconato de clorhexidina al 0,12% frente a cepas de Streptococcus mutans in vitro.
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Antibacterial efficacy of traditional toothpaste vs phytotherapy toothpaste against Streptococcus mutans in vitro
(Universidad Privada Norbert Wiener, 2021-10-26) Quispe Poccohuanca,Lisette Jadira; Vilchez Bellido, Dina
The objective of this research was to determine the antibacterial efficacy of traditional toothpaste versus phytotherapeutic toothpaste against Streptococcus mutans. An in vitro experimental study was conducted using the Kirby-Bauer technique with the agar diffusion method. The sample consisted of 20 blood agar Petri dishes inoculated with the Streptococcus mutans ATC 25175 strain. On their surface, 40 antibiogram discs impregnated with supernatants of traditional and phytotherapeutic toothpastes were placed, and the inhibition halo formation was evaluated after 48 hours. Results: Both toothpastes exhibited antibacterial efficacy against Streptococcus mutans. The phytotherapeutic toothpaste showed greater inhibition efficacy, with an inhibition halo of 14.21 mm, compared to the traditional toothpaste, which had an inhibition halo of 10.57 mm, with a statistically significant difference (p < 0.05). Conclusion: The phytotherapeutic toothpaste demonstrated greater antibacterial efficacy against Streptococcus mutans.
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Antimicrobial activity of dental pastes with and without triclosan on Streptococcus mutans ATCC 25175 strains: comparative in vitro study, Lima 2018
(Universidad Privada Norbert Wiener, 2018-10-16) Trujillo Falcón, Soledad Violeta; Garavito Chang, Enna Lucila
The objective of this research was to determine the antimicrobial activity of toothpaste with and without triclosan on Streptococcus mutans. The sample size was determined by sample size calculation, and the strain studied was of the ATCC 25175 type. The sample consisted of 40 Petri dishes, each with 4 wells of 5 millimeters in diameter, where 0.05 milliliters of toothpaste was placed, and the evaluation was done at 24 and 48 hours. The study was experimental in vitro. Data were evaluated using the SPSS v22 statistical package, employing the ANOVA test and Tukey’s multiple comparisons test. The results showed that toothpaste with triclosan exhibited an antimicrobial activity of 41.05 ± 3.58 mm on Streptococcus mutans at 24 hours and 39.75 ± 3.32 mm at 48 hours, while toothpaste without triclosan showed antimicrobial activity of 39.00 ± 4.05 mm, 37.8 ± 5.15 mm, and 39.15 ± 3.87 mm at 24 hours and 36.80 ± 4.14 mm, 35.85 ± 4.84 mm, and 37.45 ± 3.72 mm at 48 hours. It was also found that at 24 hours, toothpaste with and without triclosan did not present statistically significant differences. It is concluded that both toothpaste with and without triclosan are effective antimicrobial agents against Streptococcus mutans.
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Antimicrobial effect of remineralizing toothpastes against streptococcus mutans – In Vitro study, Lima 2020.
(Universidad Privada Norbert Wiener, 2020-12-28) Lazaro Diego, Miriam Luz; Céspedes Porras, Jacqueline
The objective of this study was to evaluate the antimicrobial effect of two remineralizing toothpaste formulations compared to a fluoride toothpaste against the ATTCC 25175 strain of Streptococcus mutans through microbiological tests. An in vitro experimental design was used to observe the antimicrobial effect of three toothpaste formulations: Group 1 with a nanocomplex of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP), Group 2 with Hydroxyapatite, and Group 3 with Fluoride. The inhibition halos were measured using the disk diffusion method (Kirby-Bauer method) on agar plates previously inoculated with the bacterial strain at 24 and 48 hours. Twenty samples were analyzed per group, and the data were recorded in a previously validated data collection form. Results: The two remineralizing toothpaste formulations, Group 1 with CPP-ACP and Group 2 with Hydroxyapatite, showed antimicrobial effects against the ATTCC 25175 strain of Streptococcus mutans at 24 hours, with mean inhibition halos of 12.60 and 11.65, respectively. At 48 hours, antimicrobial effects were observed in all three groups, with mean inhibition halos of 12.80 for Group 1, 12.0 for Group 2, and 10.20 for Group 3. A significant difference was found between the two measurements for all groups (p=0.000, p<0.05). Conclusion: All three toothpaste formulations evaluated exhibited antimicrobial effects against the ATTCC 25175 strain of Streptococcus mutans at 48 hours, with the remineralizing pastes showing slightly superior effects.
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Citotoxicidad y efecto bactericida de la Matricaria Chamomilla sobre el Streptococcus Mutans- in vitro
(Universidad Privada Norbert Wiener, 2025-11-05) Valdivieso Ramirez, Jhenifer Milytza; Trucios Saldarriaga, Karina Milagritos
Se analizó tanto la acción bactericida como la citotoxicidad del aceite esencial de Matricaria chamomilla al 25 %, en comparación con el digluconato de clorhexidina al 0,12 % y al agua destilada como control negativo frente a Streptococcus mutans., en condiciones in vitro. Se utilizó la técnica de Kirby-Bauer para medir la sensibilidad bacteriana y Microscopia óptica, técnica para visualizar bacterias u otros microorganismos en el microscopio y analizar la viabilidad celular. Se aplicaron discos con manzanilla al 25 %, clorhexidina al 0,12 % y agua destilada (control negativo) utilizando 15 placas para efecto bactericida y 4 cámaras de Neubauer para efecto citotoxicidad exponiendo tejido celular a la manzanilla durante 5, 15 y 30 minutos, simulando el uso real de un enjuague bucal, evaluando el daño celular. Los datos indicaron que la clorhexidina tuvo mayor efecto bactericida a las 24 y 72 horas, con halos de inhibición significativamente mayores (p<0,001) que la manzanilla. No obstante, el aceite esencial también evidenció un efecto antibacteriano. En cuanto a la citotoxicidad, la manzanilla mostró menor citotoxicidad que la clorhexidina en todos los tiempos (p<0,05). En conclusión, el aceite esencial de Matricaria chamomilla al 25 % mostró acción bactericida y citotóxica contra Streptococcus mutans, destacando posible opción natural.
Acceso abierto
Efecto antibacteriano del extracto de las semillas del citrus aurantifolia swingle (limon sutil) a diferentes concentraciones sobre el streptococcus mutans
(Universidad Privada Norbert Wiener, 2025-04-25) Arellano Brandán, Ruilita Adeli; Enciso Lacunza, Jorge Antonio
Este trabajo tuvo como propósito determinar el efecto antibacteriano del extracto de las semillas de citrus aurantifolia swingle (limón sutil) a diferentes concentraciones sobre el streptococcus mutans, a las 24, 48 y 72 horas. Se utilizó una metodología experimental, cuantitativa prospectiva longitudinal, con una muestra 10 placas petri por grupo de tratamiento (100%, 75%, 50% y grupo control), siendo un total de 40 placas. Los resultados revelaron que en las 24, 48 y 72 horas frente a Streptococcus mutans ATCC 25175 las semillas de Citrus aurantifolia al 100% presentaron promedio de (28,91 ± 4,958mm) (28,79 ± 4,956mm) (28,67 ± 4,959mm) respectivamente, al 75% (19,64 ± 3,326mm) (19,53 ± 3,322mm) (19,39 ± 3,306mm) respectivamente y al 50% (12,94 ± 1,675mm) (12,81 ± 1,657mm) (28,67 ± 4,959mm) respectivamente y el control positivo de ensayo, digluconato de clorhexidina al 0,12%, tuvo un promedio de 22,10 ± 1,031mm; 21,97 ± 1,059mm y 21,86 ± 1,050mm en los tres tiempos evaluados respectivamente; respecto al Streptococcus mutans se puede decir que es sumamente sensible (+++) frente al Citrus aurantifolia al 100% y digluconato de clorhexidina al 0,12%; asimismo, es muy sensible (++) y sensible (+) a Citrus aurantifolia al 75% y al 50%, respectivamente según dicha escala de sensibilidad antes mencionada a las 24, 48 y 72 horas de estudio. Por otro lado, el análisis estadístico demostró efecto antibacteriano frente a Streptococcus mutans (p=0.00) al 95 % de confianza. Se concluye que existe efecto antibacteriano del extracto de las semillas de Citrus aurantifolia swingle (limón sutil) a diferentes concentraciones sobre el Streptococcus mutans, a las 24, 48 y 72 horas.
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Efecto bactericida del aceite esencial de la Matricaria chamomilla en concentración al 25%, 35% y 50% en comparación a la clorhexidina al 0.12% sobre el Streptococcus mutans, in vitro
(Universidad Privada Norbert Wiener, 2025-10-07) Andrade Luna, Ruben Antonio; Trucios Saldarriaga, Karina Milagritos
El objetivo de esta investigación fue comparar el efecto bactericida del aceite esencial de Matricaria chamomilla (manzanilla) en concentraciones del 25%, 35% y 50% con la clorhexidina al 0.12% sobre Streptococcus mutans, bacteria principal en la etiología de la caries dental. Se empleó un diseño experimental, cuantitativo, prospectivo y comparativo in vitro. La muestra consistió en 15 placas Petri inoculadas con S. mutans, distribuidas aleatoriamente, a las cuales se aplicaron discos impregnados con las distintas concentraciones del aceite esencial, clorhexidina y agua destilada como control negativo. El método utilizado fue la técnica de difusión en disco (Kirby-Bauer), midiendo los halos de inhibición a las 24 horas, 72 horas y 7 días. Los datos fueron procesados mediante análisis estadístico ANOVA, considerando un nivel de significancia de p ≤ 0.05. Los resultados evidenciaron que todas las concentraciones de manzanilla inhibieron el crecimiento bacteriano, con mejor desempeño al 50% (8,21 mm a 24 h; 7,81 mm a 72 h; 7,47 mm a 7 días), seguido del 35% y 25%. Se concluyo que la clorhexidina mostró halos significativamente mayores en todos los tiempos (25,95 mm; 24,94 mm; 24,48 mm; p<0,05).