Efecto antibacteriano del extracto de las semillas del citrus aurantifolia swingle (limon sutil) a diferentes concentraciones sobre el streptococcus mutans

Abstract

Este trabajo tuvo como propósito determinar el efecto antibacteriano del extracto de las semillas de citrus aurantifolia swingle (limón sutil) a diferentes concentraciones sobre el streptococcus mutans, a las 24, 48 y 72 horas. Se utilizó una metodología experimental, cuantitativa prospectiva longitudinal, con una muestra 10 placas petri por grupo de tratamiento (100%, 75%, 50% y grupo control), siendo un total de 40 placas. Los resultados revelaron que en las 24, 48 y 72 horas frente a Streptococcus mutans ATCC 25175 las semillas de Citrus aurantifolia al 100% presentaron promedio de (28,91 ± 4,958mm) (28,79 ± 4,956mm) (28,67 ± 4,959mm) respectivamente, al 75% (19,64 ± 3,326mm) (19,53 ± 3,322mm) (19,39 ± 3,306mm) respectivamente y al 50% (12,94 ± 1,675mm) (12,81 ± 1,657mm) (28,67 ± 4,959mm) respectivamente y el control positivo de ensayo, digluconato de clorhexidina al 0,12%, tuvo un promedio de 22,10 ± 1,031mm; 21,97 ± 1,059mm y 21,86 ± 1,050mm en los tres tiempos evaluados respectivamente; respecto al Streptococcus mutans se puede decir que es sumamente sensible (+++) frente al Citrus aurantifolia al 100% y digluconato de clorhexidina al 0,12%; asimismo, es muy sensible (++) y sensible (+) a Citrus aurantifolia al 75% y al 50%, respectivamente según dicha escala de sensibilidad antes mencionada a las 24, 48 y 72 horas de estudio. Por otro lado, el análisis estadístico demostró efecto antibacteriano frente a Streptococcus mutans (p=0.00) al 95 % de confianza. Se concluye que existe efecto antibacteriano del extracto de las semillas de Citrus aurantifolia swingle (limón sutil) a diferentes concentraciones sobre el Streptococcus mutans, a las 24, 48 y 72 horas.

The purpose of this work was to determine the antibacterial effect of citrus aurantifolia swingle (subtle lemon) seed extract at different concentrations on streptococcus mutans at 24, 48 and 72 hours. A longitudinal prospective quantitative experimental methodology was used, with a sample of 10 petri dishes per treatment group (100%, 75%, 50% and control group), for a total of 40 dishes. The results revealed that at 24, 48 and 72 hours against Streptococcus mutans ATCC 25175 the seeds of Citrus aurantifolia at 100% showed an average of (28.91 ± 4.958mm) (28.79 ± 4.956mm) (28.67 ± 4.959mm) (28.67 ± 4.959mm) respectively, at 75% (19.64 ± 3, 326mm) (19.53 ± 3.322mm) (19.39 ± 3.306mm) respectively and at 50% (12.94 ± 1.675mm) (12.81 ± 1.657mm) (28.67 ± 4.959mm) respectively and the positive test control, 0.12% chlorhexidine digluconate, averaged 22.10 ± 1.031mm; 21.97 ± 1.059mm and 21.86 ± 1.050mm in the three times evaluated respectively; with respect to Streptococcus mutans it can be said that it is highly sensitive (+++) to Citrus aurantifolia at 100% and 0.12% chlorhexidine digluconate; it is also very sensitive (++) and chlorhexidine digluconate at 0.12%; it is also very sensitive (++) and sensitive (+) to Citrus aurantifolia at 75% and 50%, respectively, according to the aforementioned sensitivity scale at 24, 48 and 72 hours of study. On the other hand, the statistical analysis showed an antibacterial effect against Streptococcus mutans (p=0.00) at 95 % confidence. It is concluded that there is an antibacterial effect of Citrus aurantifolia swingle (subtle lemon) seed extract at different concentrations on Streptococcus mutans at 24, 48 and 72 hours.